Clinical cardiac alterations and hemostatic toxicities caused by scorpion (Tityus discrepans) venom and its purified fractions on zebrafish (Danio rerio) larvae

IF 0.1 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Investigacion clinica Pub Date : 2021-11-29 DOI:10.22209/ic.v62n4a04

Aurora Alvarez, M. Álvarez, L. Perdomo, A. Rodríguez-Acosta

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Abstract

Envenomation by the Venezuelan scorpion Tityus discrepans is typified by local and systemic alterations. The current work investigated the in vivo hemostatic processes, cardiac dysfunction and tissue destruction triggered by Tityus discrepans purified toxins 1 (3 kDa) and 2 (5 kDa) fractions. These fractions were obtained by C-18-HPLC chromatography. The hemostatic and cardiovascular toxicities in zebrafish of both fractions was assessed by means of specific phenotypic expressions and larvae behavior at 5, 15, 30, 40 and 60 min post-venom-treatment. The Tityus discrepans venom fractions 1 and 2 produced disseminated intravascular coagulation (presence of thrombus) in the central vein of the larva, heart rate/rhythm alterations, and necrotic events in more than 90% of all the larvae under their action. The outcomes have established the potential hemostatic and cardiovascular toxicities by Tityus discrepans venom, alerting on the possibility of cardiovascular injuries and thromboembolism in humans after scorpion stings envenomation.

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蝎毒及其纯化组分对斑马鱼幼虫的临床心脏改变和止血毒性

委内瑞拉蝎子Tityus离散的包膜以局部和系统的改变为代表。目前的工作研究了由Tityus离散纯化的毒素1（3kDa）和2（5kDa）级分引发的体内止血过程、心脏功能障碍和组织破坏。这些级分通过C-18-HPLC色谱法获得。通过毒液处理后5、15、30、40和60分钟的特异性表型表达和幼虫行为来评估两种组分在斑马鱼中的止血和心血管毒性。Tityus分散的毒液组分1和2在幼虫的中心静脉中产生弥散性血管内凝血（存在血栓），在其作用下，90%以上的幼虫出现心率/节律改变和坏死事件。研究结果已经确定了Tityus离散毒液的潜在止血和心血管毒性，提醒人们在蝎子蜇伤环境后可能发生心血管损伤和血栓栓塞。

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