M. Khodaei, Y. Amanzadeh, M. Faramarzi, M. Pirali-Hamedani, H. Adhami
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引用次数: 4
Abstract
Background and objectives: Dracocephalum species are mentioned in Iranian traditional medicine for enhancement of cognitive performance. In the present study, the acetyl cholinesterase inhibitory and butyryl cholinesterase inhibitory activities as well as the anti-oxidant and anti-tyrosinase effects of three Iranian Dracocephalum species (D. kotschyi, D. multicaule, D. polychaetum was analyzed). Methods: The extractions were performed stepwise with hexane, chloroform, ethyl acetate (EtOAC), methanol (MeOH) and water. AChE and BChE inhibitory properties were measured by a microplate assay. Total phenolic content of all extracts were also evaluated and anti-oxidant activities of the extracts were assessed using DPPH, FRAP assays. Tyrosinase inhibitory activity was measured using the modified dopachrome method with L-DOPA as the substrate. Results: The results showed that the EtOAc extract of D. multicaule and MeOH extract of D. kotschyi were the most active anti-oxidant and anti-tyrosinase extracts which showed the highest amounts of phenolic compounds. Dracocephalum multicaule demonstrated the most considerable activity in AChE inhibition and D. polychaetum the highest activity in BChE inhibition. The aqueous extract of D. multicaule inhibited both AChE and BChE. Conclusion: Dracocephalum multicaule can be suggested as a proper natural candidate for improvement of cognitive disorders.
背景和目的:在伊朗传统医学中提到了龙头属植物,以增强认知能力。本研究分析了3种伊朗龙头草(D. kotschyi, D. multicaule, D. polychaetum)的乙酰胆碱酯酶抑制活性和丁酰胆碱酯酶抑制活性,以及抗氧化和抗酪氨酸酶作用。方法:采用正己烷、氯仿、乙酸乙酯(EtOAC)、甲醇(MeOH)和水进行分步提取。用微孔板法测定AChE和BChE的抑制性能。采用DPPH、FRAP测定各提取物的总酚含量和抗氧化活性。以左旋多巴为底物,采用改良多巴色法测定酪氨酸酶抑制活性。结果:结果表明,多孔竹叶乙酸乙酯提取物和多孔竹叶甲醇提取物的抗氧化活性和抗酪氨酸酶活性最高,酚类化合物含量最高。对乙酰胆碱酯酶(AChE)的抑制作用最强,对BChE的抑制作用最强。水提物对AChE和BChE均有抑制作用。结论:龙脑可作为改善认知障碍的天然候选药物。