FORMULASI MEDIA ALTERNATIF DARI KULIT SINGKONG, PISANG, DAN WHEY UNTUK Lactobacillus bulgaricus

Jurnal Teknologi dan Industri Pangan Pub Date : 2021-07-01 DOI:10.6066/jtip.2021.32.1.52

M. Wachid, Yasmin Nurinbaity, Noor Harini, Elfi Anis Saati, Vritta Amroini Wahyudi

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引用次数: 1

Abstract

De Man Rogose and Sharpe (MRS) growth medium is a specific growth medium for lactic acid bacteria (LAB) but has a relatively expensive price. This can be overcome by making alternative media from waste materials. This study used Lactobacilus bulgaricus to represent LAB analyzed for growth. The research aims to formulate alternative media from the waste of cassava peels, banana peels, and tofu whey; determine the best formulation based on the growth parameters of L. bulgaricus; apply the L. bulgaricus in soygurt making and analyze the quality of the resulting yogurt. The results showed the composition of cassava peel: banana peel (75:25 w/w) with the addition of urea 1.5% as the best formu-lation. This formula yields a total LAB of 8.6x106 CFU/mL. The total LAB in the alternative medium was higher than that in MRS control at 1.7x106 CFU/mL. Soygurt produced from LAB as a result of growth from the best media [cassava peel+banana peel (75:25) with the addition of 1.5% urea] resulted in a fat content of 0.9%, total titrated acid 0.6%, protein 8.1 %, pH 4.7, viscosity 14.2 dPas, total dissolved solids 9.7°Brix, brightness (L) 64.3, and yellow-blue (b +) 10.1. These fat content, total titrated acid, and protein are in accordance with SNI for yogurt 2981: 2009.

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De Man Rogose and Sharpe（MRS）生长培养基是乳酸菌（LAB）的特异性生长培养基，但价格相对昂贵。这可以通过用废料制造替代介质来克服。本研究使用保加利亚乳杆菌作为生长分析实验室。该研究旨在从木薯皮、香蕉皮和豆腐乳清的废料中配制替代培养基；根据保加利亚乳杆菌的生长参数确定最佳配方；将保加利亚乳杆菌应用于豆制品中，并对所得酸奶的质量进行分析。结果表明，木薯皮的成分为：香蕉皮（75:25w/w），尿素添加量为1.5%为最佳配方。该配方产生8.6x106 CFU/mL的总LAB。替代培养基中的总LAB高于MRS对照，为1.7x106CFU/mL。LAB生产的大豆是在最佳培养基[木薯皮+香蕉皮（75:25），添加1.5%尿素]中生长的结果，其脂肪含量为0.9%，总滴定酸为0.6%，蛋白质为8.1%，pH为4.7，粘度为14.2dPas，总溶解固体9.7°Brix，亮度（L）64.3，黄蓝（b+）10.1。这些脂肪含量、总滴定酸和蛋白质符合酸奶2981:2009的SNI。

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来源期刊

Jurnal Teknologi dan Industri Pangan

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审稿时长

24 weeks