Implications of ectomycorrhizal inoculation for drought stress tolerance of Atlas cedar (Cedrus atlantica (Endl.) Carrière) seedlings

IF 1.5 4区农林科学 Q2 FORESTRY

New Zealand Journal of Forestry Science Pub Date : 2021-07-09 DOI:10.33494/NZJFS512021X131X

Hamida Gaba Chahboub, Mohamed Sghir Lamhamedi, O. Abrous-Belbachir

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引用次数: 0

Abstract

Background: Ectomycorrhizal inoculation is a promising strategy to minimise the initial transplant shock and increase plant survival and growth during the first years of out-planting in the field. The aim of this research was to investigate the effect of sporal inoculum of three ectomycorrhizal fungi: Cortinarius cedretorum, Amanita vaginata and Inocybe geophylla on tolerance levels of Atlas cedar (Cedrus atlantica (Endl.) Carrière) seedlings subjected to applied drought stress in nursery conditions. Methods: Carpophores, seeds and organic forest soil were collected under pure stands of Atlas cedar. After fifteen months of growth, seedlings were subjected to drought stress by withholding water for thirty days; we assessed morphological and physiological variables of all seedling batches (inoculated and uninoculated, controlled and stressed seedlings) Results: All roots of inoculated stressed seedlings were mycorrhizal. The mycorrhization rates were 67%, 64.6% and 53.6% for stressed seedlings inoculated with Cortinarius cedretorum, Amanita vaginata, Inocybe geophylla, respectively. This root mycorrhization was accompanied by a significant improvement in seedling growth, especially height and length of the main root (10.2 cm, 52 cm) reached in stressed seedlings inoculated with Cortinarius cedretorum. There was a significant increase in relative water content, total chlorophyll, carotenoids, soluble sugars and starch, superoxide dismutase and ascorbate peroxydase enzyme activities in inoculated stressed seedlings compared with uninoculated seedlings. Conclusions: Inoculation of Atlas cedar seedlings with spores of ectomycorrhizal fungi remains a very effective alternative for improving growth and the morphological and physiological status of seedlings under drought conditions. Cortinarius cedretorum appears to be consistently advantageous followed by Amanita vaginata and Inocybe geophylla.

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外生菌根接种对阿特拉斯雪松(Cedrus atlantica, Endl.)耐旱性的影响卡里埃)幼苗

背景：外生菌根接种是一种很有前途的策略，可以最大限度地减少最初的移植冲击，并在田间种植的头几年提高植物的存活率和生长。本研究的目的是研究三种外生菌根真菌：Cortinarius cedretorum、Amanita vaginata和Inocybe geophylla的孢子接种物对阿特拉斯雪松（Cedrus atlantica（Endl.）Carrière）幼苗在苗圃条件下承受干旱胁迫的耐受水平的影响。方法：在阿特拉斯雪松纯林下采集载体、种子和有机林地土壤。在生长15个月后，幼苗通过保持水分30天而受到干旱胁迫；我们评估了所有幼苗批次（接种和未接种、对照和胁迫幼苗）的形态和生理变量。结果：接种胁迫幼苗的所有根都是菌根。用Cortinarius cedretorum、Amanita vaginata和Inocybe geophylla接种胁迫幼苗的菌根化率分别为67%、64.6%和53.6%。这种根菌根伴随着幼苗生长的显著改善，特别是在接种了玉髓的胁迫幼苗中，主根的高度和长度（10.2厘米，52厘米）达到了显著改善。与未接种的幼苗相比，接种胁迫的幼苗的相对含水量、总叶绿素、类胡萝卜素、可溶性糖和淀粉、超氧化物歧化酶和抗坏血酸过氧化物酶活性显著增加。结论：在干旱条件下，用外生菌根真菌孢子接种阿特拉斯雪松幼苗对于改善幼苗的生长和形态生理状况仍然是一种非常有效的选择。Cortinarius cedretorum似乎一直是有利的，其次是Amanita vaginata和Inocybe geophylla。

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来源期刊

New Zealand Journal of Forestry Science FORESTRY-

CiteScore

2.20

自引率

13.30%

发文量

审稿时长

39 weeks

期刊介绍： The New Zealand Journal of Forestry Science is an international journal covering the breadth of forestry science. Planted forests are a particular focus but manuscripts on a wide range of forestry topics will also be considered. The journal''s scope covers forestry species, which are those capable of reaching at least five metres in height at maturity in the place they are located, but not grown or managed primarily for fruit or nut production.