Resolving the phylogenetic relationship between Parmotrema crinitum and Parmotrema perlatum populations

IF 1.6 4区生物学 Q4 MYCOLOGY

Lichenologist Pub Date : 2022-07-01 DOI:10.1017/S0024282922000147

Ayoub Stelate, Ruth Del-Prado, David Alors, H. Tahiri, P. Divakar, A. Crespo

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引用次数: 2

Abstract

Abstract The widespread species Parmotrema crinitum (Ach.) M. Choisy and Parmotrema perlatum (Huds.) M. Choisy are mainly distinguished by their reproductive strategies. While P. crinitum propagates by isidia, P. perlatum produces soredia. In this study, we aim to evaluate the phylogenetic relationship between both species and to critically examine their species boundaries. To this purpose, 46 samples belonging to P. crinitum and P. perlatum were used in our analysis, including 22 for which we studied the morphology and chemistry, before extracting their DNA. We used 35 sequences of the internal transcribed spacer region of nuclear ribosomal DNA (ITS) of Parmotrema perlatum from Europe and Africa (20 of which were newly generated), and 11 of Parmotrema crinitum from Europe, North America and North Africa (two newly generated). Additionally, 28 sequences of several species from Parmotrema were included in the ITS dataset. The ITS data matrix was analyzed using different approaches, such as traditional phylogeny (maximum likelihood and Bayesian analyses), genetic distances, automatic barcode gap discovery (ABGD) and the coalescent-based method poisson tree processes (PTP), in order to test congruence among results. Our results indicate that all samples referred to P. crinitum and P. perlatum nested in a well-supported monophyletic clade, but phylogenetic relationships among them remain unresolved. Delimitations inferred from PTP, ABGD and genetic distance analyses were comparable and suggested that P. crinitum and P. perlatum belong to the same lineage. Interestingly, two samples of P. perlatum separate in a different monophyletic clade, which is supported as a different lineage by all the analyses.

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解决皱纹Parmotrema和perlatum种群之间的系统发育关系

摘要广泛分布的海百合和海百合主要区别于它们的繁殖策略。而海百合通过isidia繁殖，perlatum产生soredia。在这项研究中，我们旨在评估两个物种之间的系统发育关系，并严格检查它们的物种边界。为此，在我们的分析中使用了46个属于P.crinitum和P.perlatum的样品，其中22个样品在提取它们的DNA之前进行了形态和化学研究。我们使用了来自欧洲和非洲的perlatum Parmotrema核核糖体DNA（ITS）的35个内部转录间隔区序列（其中20个是新产生的），以及来自欧洲、北美和北非的褶皱Parmotremma的11个（两个是新生成的）。此外，ITS数据集中还包括来自Parmotrema的几个物种的28个序列。使用不同的方法分析ITS数据矩阵，如传统的系统发育（最大似然和贝叶斯分析）、遗传距离、自动条形码间隙发现（ABGD）和基于联合的方法泊松树过程（PTP），以测试结果之间的一致性。我们的研究结果表明，所有涉及P.crinitum和P.perlatum的样本都嵌套在一个得到充分支持的单系分支中，但它们之间的系统发育关系仍未解决。根据PTP、ABGD和遗传距离分析推断的界线具有可比性，表明海百合和perlatum属于同一谱系。有趣的是，两个P.perlatum样本在不同的单系分支中分离，所有分析都支持这是一个不同的谱系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Lichenologist 生物-真菌学

CiteScore

2.90

自引率

14.30%

发文量

审稿时长

6.0 months

期刊介绍： The Lichenologist is the premier scientific journal devoted exclusively to the study of lichens worldwide. As the leading forum for the dissemination of new concepts and topical reviews, The Lichenologist reaches more scientists concerned with the study of lichens and lichen symbionts than any other single journal. All aspects of lichenology are considered including systematics and phylogenetics; molecular biology; ultrastructure, anatomy and morphology; secondary chemistry, effects of pollutants and use as bioindicators; biogeography. In addition to standard length research papers, the journal also publishes Short Communications and Book Reviews. A monthly issue may occasionally be devoted to papers deriving from a symposium.