Presence of Senecavirus A in pork sold in the United States

IF 0.7 4区农林科学 Q3 Agricultural and Biological Sciences

Journal of Swine Health and Production Pub Date : 2019-03-01 DOI:10.54846/jshap/1107

Vlad Petrovan, Ying Fang, R. Rowland

引用次数: 0

Abstract

Objective: To estimate the prevalence and concentration of Senecavirus A (SVA) in meat sold at retail. Materials and methods: A total of 190 meat samples derived from 25 processing locations in 13 states were purchased through retail sources. The presence of virus in samples of muscle obtained from each package was assessed by polymerase chain reaction (PCR) amplification of SVA nucleic acid. A standard curve was constructed to estimate the concentration of viable virus in PCR-positive samples. Results: Two of the 190 meat samples (1.1%) were positive for SVA nucleic acid, but negative for virus by virus isolation. The amount of virus in the PCR-positive samples was estimated to be less than 14 virions/g of muscle. Implications: The low prevalence of SVA in the 190 retail-meat samples analyzed in this study, combined with a low concentration of SVA nucleic acid in the two SVA-positive samples, suggest a low risk for transmitting SVA through retail meat.

查看原文本刊更多论文

在美国销售的猪肉中发现塞内卡病毒A

目的：估计零售肉类中塞涅卡病毒A（SVA）的流行率和浓度。材料和方法：通过零售渠道购买了来自13个州25个加工点的190份肉类样本。通过SVA核酸的聚合酶链式反应（PCR）扩增来评估从每个包装获得的肌肉样品中是否存在病毒。构建了一条标准曲线来估计PCR阳性样本中活病毒的浓度。结果：190份肉类样品中有2份（1.1%）SVA核酸阳性，但病毒分离呈阴性。PCR阳性样本中的病毒量估计小于14个病毒粒子/g肌肉。含义：本研究中分析的190份零售肉类样本中SVA的低流行率，加上两份SVA阳性样本中的SVA核酸浓度较低，表明通过零售肉类传播SVA的风险较低。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Swine Health and Production 农林科学-兽医学

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

>36 weeks

期刊介绍： The Journal of Swine Health & Production (JSHAP) is an open-access and peer-reviewed journal published by the American Association of Swine Veterinarians (AASV) since 1993. The aim of the journal is the timely publication of peer-reviewed papers with a scope that encompasses the many domains of applied swine health and production, including the diagnosis, treatment, management, prevention and eradication of swine diseases, welfare & behavior, nutrition, public health, epidemiology, food safety, biosecurity, pharmaceuticals, antimicrobial use and resistance, reproduction, growth, systems flow, economics, and facility design. The journal provides a platform for researchers, veterinary practitioners, academics, and students to share their work with an international audience. The journal publishes information that contains an applied and practical focus and presents scientific information that is accessible to the busy veterinary practitioner as well as to the research and academic community. Hence, manuscripts with an applied focus are considered for publication, and the journal publishes original research, brief communications, case reports/series, literature reviews, commentaries, diagnostic notes, production tools, and practice tips. All manuscripts submitted to the Journal of Swine Health & Production are peer-reviewed.