Phylogenetic inference applied to germplasm bank characterization and interspecific breeding in passionfruit

IF 1.5 4区农林科学 Q2 Agricultural and Biological Sciences

Crop Breeding and Applied Biotechnology Pub Date : 2021-05-21 DOI:10.1590/1984-70332021V21N1A12

P. Inglis, G. Bellon, N. Junqueira, F. Faleiro, M. E. Ferreira

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引用次数: 0

Abstract

Abstract Interspecific crosses between the more than 520 Passiflora species may or may not be compatible. The sequences ITS, matK, psbA-trnH, rbcL and trnL-F were used to confirm species identity and to estimate the genetic distances among 48 Passiflora accessions of a germplasm bank. Twenty species were used for crosses within and between distinct Passiflora subgenera. The phylogenetic resolution based on ITS data was superior to that of any single chloroplast marker, recommending it as a DNA barcode for this genus. However, the tree topology based on Bayesian phylogenetic analysis using the combination of the four chloroplast markers possessed greater support for subclades within the subgenus Passiflora, which contains more species of interest for breeders. We could not identify a clear cutoff value of pairwise Kimura two-parameter (K2P) distances to predict success or failure of crosses. Rather, the clustering of species pairs together within the same or closely related phylogenetic subclades predicted the probability of wide cross compatibility more reliably.

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系统发育推断在百香果种质库鉴定和种间育种中的应用

摘要520多种西番莲之间的种间杂交可能是相容的，也可能不是相容的。利用ITS、matK、psbA-trnH、rbcL和trnL-F序列对一个种质库的48份西番莲材料进行了物种鉴定和遗传距离估计。20个物种被用于不同西番莲亚属内部和之间的杂交。基于ITS数据的系统发育分辨率优于任何单一叶绿体标记，推荐将其作为该属的DNA条形码。然而，基于贝叶斯系统发育分析的树状拓扑结构使用了四个叶绿体标记的组合，对西番莲亚属中的子属有更大的支持，西番莲亚属包含了更多育种家感兴趣的物种。我们无法确定成对Kimura双参数（K2P）距离的明确截止值来预测杂交的成功或失败。相反，在相同或密切相关的系统发育子系统中，物种配对的聚类更可靠地预测了广泛交叉兼容性的可能性。

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来源期刊

Crop Breeding and Applied Biotechnology 农林科学-农艺学

CiteScore

2.40

自引率

13.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The CBAB – CROP BREEDING AND APPLIED BIOTECHNOLOGY (ISSN 1984-7033) – is the official quarterly journal of the Brazilian Society of Plant Breeding, abbreviated CROP BREED APPL BIOTECHNOL. It publishes original scientific articles, which contribute to the scientific and technological development of plant breeding and agriculture. Articles should be to do with basic and applied research on improvement of perennial and annual plants, within the fields of genetics, conservation of germplasm, biotechnology, genomics, cytogenetics, experimental statistics, seeds, food quality, biotic and abiotic stress, and correlated areas. The article must be unpublished. Simultaneous submitting to another periodical is ruled out. Authors are held solely responsible for the opinions and ideas expressed, which do not necessarily reflect the view of the Editorial board. However, the Editorial board reserves the right to suggest or ask for any modifications required. The journal adopts the Ithenticate software for identification of plagiarism. Complete or partial reproduction of articles is permitted, provided the source is cited. All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY. All articles are published free of charge. This is an open access journal.