Phytochemical Properties and Antimicrobial Activity of Natural Colorant Extracted from Mesocarp and Exocarp of Cocos nucifera

R. Hassan, Nur Asma Fhadhila Zulrushdi, A. M. Yusoff, Nurhafizah Ibrahim, Norakma Mohd Nor
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Abstract

Phytochemical analysis was carried out on the natural colorant extracted using the microwave-assisted extraction which performed at a microwave power of 300 watts for 2 minutes. The pigment compound was extracted from mesocarp and mesocarp of Cocos nucifera by using 0.1 M NaOH as extracting agent. The qualitative analysis that was carried out for these natural colorants confirmed that flavonoids, terpenoids, cardiac glycosides and phenol/tannins were present in both plant parts (mesocarp and exocarp) tested whereas alkaloids, saponins, steroids and anthraquinone were not detected. Quantitative analysis showed that tannin content was significantly (p < 0.05) higher (134.82 mg TAE/g) in the mesocarp extract compared to the exocarp extract (33.90 mg TAE/g). The amount of flavonoids was low in both extracts (mesocarp: 15.48 mg QE/g and exocarp: 28.45 mg QE/g, respectively). The antimicrobial study discovered that the mesocarp and the exocarp extract were not effective as antibacterial agents.
椰子中果皮和外果皮天然色素的理化性质及抗菌活性
采用微波辅助提取法,在300瓦微波功率下提取2分钟,对提取的天然着色剂进行植物化学分析。以0.1 M NaOH为萃取剂,从椰子中果皮和中果皮中提取色素化合物。对这些天然着色剂进行定性分析,证实黄酮类、萜类、心糖苷类和酚/单宁类均存在于所测试的植物中果皮和外果皮中,而未检测到生物碱、皂苷、类固醇和蒽醌类。定量分析表明,中果皮栲胶单宁含量(134.82 mg TAE/g)显著高于外果皮栲胶(33.90 mg TAE/g) (p < 0.05)。两种提取物的总黄酮含量均较低(中果皮:15.48 mg QE/g,外果皮:28.45 mg QE/g)。抗菌研究发现,中果皮和外果皮提取物作为抗菌药物效果不佳。
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