Targeting ‘immunogenic hotspots’ in Dengue and Zika virus: an in silico approach to a common vaccine candidate

IF 2.1 4区 医学 Q3 VIROLOGY
Dhrubajyoti Mahata, Debangshu Mukherjee, Kheerthana Duraivelan, Vanshika Malviya, P. Parida, G. Mukherjee
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引用次数: 0

Abstract

Aim: Dengue and Zika viruses cause significant mortality globally. Considering high sequence similarity between the viral proteins, we designed common multi-epitope vaccine candidates against these pathogens. Methods: We identified multiple T and B cell epitope-rich conserved ‘immunogenic hotspots’ from highly antigenic and phylogenetically related viral proteins and used these to design the multi-epitope vaccine (MEV) candidates, ensuring high global population coverage. Results: Four MEV candidates containing conserved immunogenic hotspots from E and NS5 proteins with the highest structural integrity could favorably interact with TLR4-MD2 complex in molecular docking studies, indicating activation of TLR-mediated immune responses. MEVs also induced memory responses in silico, hallmarks of a good vaccine candidate. Conclusion: Conserved immunogenic hotspots can be utilized to design cross-protective MEV candidates.
针对登革热和寨卡病毒的“免疫原性热点”:一种常见候选疫苗的计算机方法
目的:登革热和寨卡病毒在全球范围内造成重大死亡。考虑到病毒蛋白之间的高度序列相似性,我们设计了针对这些病原体的常见多表位候选疫苗。方法:我们从高度抗原和系统发育相关的病毒蛋白中鉴定了多个富含T和B细胞表位的保守“免疫原性热点”,并用这些来设计多表位候选疫苗(MEV),确保高全球人群覆盖率。结果:在分子对接研究中,四种含有E和NS5蛋白保守免疫原性热点的MEV候选物具有最高的结构完整性,可以与TLR4-MD2复合物有利地相互作用,表明TLR介导的免疫反应被激活。MEV还诱导了计算机的记忆反应,这是良好候选疫苗的标志。结论:保留的免疫原性热点可用于设计交叉保护性MEV候选物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Future Virology
Future Virology 医学-病毒学
CiteScore
4.00
自引率
3.20%
发文量
84
审稿时长
6-12 weeks
期刊介绍: Future Virology is a peer-reviewed journal that delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this ever-expanding area of research. It is an interdisciplinary forum for all scientists working in the field today.
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