INFLUENCE OF MESENCHYMAL STEM CELLS ON EFFICIENCY OF ANGIOGENESIS, STATE OF VASCULAR TONE, INTENSITY OF LIPID PEROXIDATION AND METABOLIC ACTIVITY OF CARDIOMYOCYTES IN EXPERIMENTAL MYOCARDIAL INFARCTION

Abstract

In contrast to medical and surgical methods of treatment of coronary heart disease and its complications, cellular cardiomyoplasty is aimed at creating new cells and stable lineages of normally functioning heart tissue. Autologous mesenchymal bone marrow stem cells are a promising source for such cardiomyoplasty. To study the effect of stem cell myocardial transplantation on the processes of its post−infarction state, acute myocardial infarction in laboratory rats was experimentally modeled with subsequent transplantation of autologous mesenchymal stem cells of bone marrow and comparative study in blood serum of cardiovyocytes metabolic activity markers, neoangiogenesis, vessel tonus as well as myocardial lipid peroxidation. The cells were intravenously injected into the necrotized myocardium and left ventricle. The study found that regardless of the method of administration, stem cell transplantation contributes to a significant increase in angiogenic factors, i.e. nitrogen oxide and endothelial growth factor, a significant decrease in vasoconstrictor endothelin−1, the level of TBA−active products and haptoglobin, enzyme activity, namely cardiac ischemia markers, increase in ceruloplasmin. All this indicates positive effects: leveling ischemia by improving myocardial perfusion due to compensatory vasodilation, limiting the rate of lipid peroxidation and stimulating antioxidant factors, improving the energy balance of the myocardium by increasing the level of energy substrates and activation of their aerobic pathways. Thus, cellular cardiomyoplasty improves metabolism and prevents the process of post−ischemic myocardial remodeling. Key words: cardiomyoplasty, mesenchymal stem cells, myocardial infarction, myocardial metabolism.