Protective effect of cycloartenyl ferulate on lipopolysaccharide induced endothelial cell apoptosis and its mechanism

Q4 Nursing

中华急诊医学杂志 Pub Date : 2019-09-10 DOI:10.3760/CMA.J.ISSN.1671-0282.2019.09.009

Jingjing Ni, R. Xu, Xiyu Yang, Hui Chen, Long-wang Chen, J. Lian, Guangju Zhao

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引用次数: 1

Abstract

Objective To investigate the effect of cycloartenyl ferulate (CF) on lipopolysaccharide (LPS)-induced apoptosis in human umbilical vein endothelial cells (HUVEC), and to explore its relative mechanism. Methods Human umbilical vein endothelial cells were cultured in vitro, and the experiment was divided into the normal group, CF group, LPS group, and LPS+CF groups at different concentrations. After the corresponding treatment of cells in each group, the cell viability and apoptosis of each group were tested by the cell counter Kit-8 (CCK-8) assay and TdT-mediated dUTP nick end labeling (TUNEL) assay. The protein expression of Bax, Bcl-2, caspase-3 and the effect of CF on the protein expression of Nrf2/HO-1 pathway were determined by Western blot. One-way ANOVA were performed in multigroup mean comparison, LSD-t test was used to compare the mean values of two samples, and P<0.05 was considered statistically significant. Results Compared with the LPS group, the survival rate of HUVECs cells in the CF group was significantly increased with different doses, and the survival rate increased significantly in the 320 μmol/L CF group [(69.85±1.2)% vs ( 100.2±1.824)%, P< 0.01]. TUNEL staining showed that compared with the LPS group, the number of apoptotic positive cells in the 320 μmol/L CF group was significantly reduced [(27.33 ± 3.40) vs (11.67 ± 2.04), P<0.01]. Compared with the LPS group, Bcl-2 protein expression level was significantly increased in the CF group at different doses (P<0.01), caspase-3 and Bax protein expression were significantly decreased (P<0.01). Nrf2 protein expression level increased significantly (P<0.01), and HO-1 protein level increased significantly (P<0.01). Conclusion CF can alleviate LPS-induced apoptosis of HUVEC, which may be related to the increase of bcl-2 expression, the decrease of caspase-3 and Bax protein expression and the activation of Nrf2/ HO-1 signaling pathway. Key words: Cycloartenyl ferulate; Lipopolysaccharide; Vascular endothelial cells; Apoptosis; Nrf2/HO-1 signaling pathway

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阿魏酸环烯酯对脂多糖诱导的内皮细胞凋亡的保护作用及其机制

目的研究阿魏酸环烯酯（CF）对脂多糖（LPS）诱导的人脐静脉内皮细胞（HUVEC）凋亡的影响，并探讨其相关机制。方法体外培养人脐静脉内皮细胞，实验分为正常组、CF组、LPS组和不同浓度的LPS+CF组。在对各组细胞进行相应处理后，通过细胞计数器Kit-8（CCK-8）测定和TdT介导的dUTP缺口末端标记（TUNEL）测定来测试各组的细胞活力和细胞凋亡。Western blot检测Bax、Bcl-2、胱天蛋白酶-3的蛋白表达以及CF对Nrf2/HO-1通路蛋白表达的影响。单因素方差分析用于多组平均值比较，LSD-t检验用于比较两个样本的平均值，P<0.05被认为具有统计学意义。结果与LPS组相比，CF组HUVECs细胞的存活率在不同剂量下显著提高，320μmol/L CF组的存活率显著提高[（69.85±1.2）%vs（100.2±1.824）%，P<0.01]，320μmol/L CF组凋亡阳性细胞数明显减少[（27.33±3.40）vs（11.67±2.04），P<0.01]。与LPS组相比，不同剂量CF组Bcl-2蛋白表达水平显著升高（P<0.01），结论CF可减轻LPS诱导的HUVEC细胞凋亡，其作用可能与bcl-2表达增加有关，胱天蛋白酶-3和Bax蛋白表达的降低以及Nrf2/HO-1信号通路的激活。关键词：阿魏酸环戊烯；脂多糖；血管内皮细胞；细胞凋亡；Nrf2/HO-1信号通路

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来源期刊

中华急诊医学杂志 Nursing-Emergency Nursing

CiteScore

0.10

自引率

0.00%

发文量

8629

期刊介绍： Chinese Journal of Emergency Medicine is the only national journal which represents the development of emergency medicine in China. The journal is supervised by China Association of Science and Technology, sponsored by Chinese Medical Association, and co-sponsored by Zhejiang University. The journal publishes original research articles dealing with all aspects of clinical practice and research in emergency medicine. The columns include Pre-Hospital Rescue, Emergency Care, Trauma, Resuscitation, Poisoning, Disaster Medicine, Continuing Education, etc. It has a wide coverage in China, and builds up communication with Hong Kong, Macao, Taiwan and international emergency medicine circles.