Extrapolation of Three Hourly Post-Mortem Interval using Some VitreousChemistry Parameters

Journal of forensics research Pub Date : 2017-01-09 DOI:10.4172/2157-7145.1000360

Agoro Es, Okoye Fbc, Onyenekwe Cc, O. Azuonwu, Ebiere Ne

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引用次数: 8

Abstract

Background Study: Vitreous humor is an inert, transparent, jelly like substance that fills the posterior four fifths of the cavity of the eyeball. It is the choice sample in post-mortem investigation and analysis. Objective of study: To determine vitreous biochemical parameters suitable for postmortem interval (PMI) extrapolation. Material and Methods: Vitreous humors were collected from the eyes of 50 dead bodies (28 males and 22 females) at the Federal Medical Center Yenagoa morgue employing Coe method. The vitreous was extracted, centrifuged and the resultant supernatant used for the biochemical analysis. The supernatants were analyzed for glucose, total protein, albumin, globulin, sodium, potassium, chloride, bicarbonate, urea and creatinine using WHO approved methods. Results: The data were statistically analyzed using SPSS. The results indicated that there was strong correlation (R-0.88) between vitreous potassium and PMI. Other parameter showed either weak or negligible correlation. The staged-three -hourly mean potassium showed a gradual increase using student t-test. Conclusion: Death within 15 hours can be extrapolated from potassium concentrations.

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用一些玻璃体化学参数推断三小时的死后间隔

研究背景:玻璃体是一种惰性的、透明的、果冻状的物质，填充眼球后腔的五分之四。是尸检调查分析的首选样本。目的:确定适用于死后时间(PMI)外推的玻璃体生化参数。材料与方法:采用Coe法从联邦医疗中心Yenagoa停尸房50具尸体(男28具，女22具)的眼睛中采集玻璃体体液。提取玻璃体，离心，所得上清用于生化分析。采用世卫组织批准的方法对上清液进行葡萄糖、总蛋白、白蛋白、球蛋白、钠、钾、氯化物、碳酸氢盐、尿素和肌酐的分析。结果:采用SPSS软件对数据进行统计学分析。结果表明，玻璃体钾与PMI有很强的相关性(R-0.88)。其他参数相关性较弱或可忽略。经学生t检验，分阶段三小时平均钾含量呈逐渐升高趋势。结论:可根据钾浓度推断15小时内死亡。

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Journal of forensics research

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