In vitro effects of PCBs and OH-PCBs on the basal and dexamethasone-modified thyroid hormone metabolism in chicken liver

IF 0.8 4区生物学 Q4 BIOLOGY

Folia Biologica-Krakow Pub Date : 2023-06-30 DOI:10.3409/fb_71-2.07

K. Kowalik, D. Katarzyńska-Banasik, Karolina Frydrych, A. Sechman

{"title":"In vitro effects of PCBs and OH-PCBs on the basal and dexamethasone-modified thyroid hormone metabolism in chicken liver","authors":"K. Kowalik, D. Katarzyńska-Banasik, Karolina Frydrych, A. Sechman","doi":"10.3409/fb_71-2.07","DOIUrl":null,"url":null,"abstract":"To assess the in vitro effect of polychlorinated biphenyls (PCBs) and their hydroxylated metabolites (OH - -PCBs) on the metabolism of thyroid hormones (THs) in chicken liver, explants of liver tissue were incubated in a medium supplemented with dexamethasone (DEX) (100 nM),\n PCB118 (dioxin-like PCB), PCB153 (non-dioxin-like PCB), 4-OH-PCB107 and 3-OH-PCB153 (0.5 × 10-8 M), and with DEX together with each of the PCBs and OH-PCBs to determine the triiodothyronine (T3) secretion, thyroxine (T4) to T3 conversion, mRNA\n expression and protein concentration of the iodothyronine deiodinases (DIO1, DIO2, DIO3), TH transporters (OATP1C1, MCT8, MCT10, LAT1) and TH receptors (THRA, THRB). The results obtained revealed that the tested PCBs and OH-PCBs interacted with and/or abolished the inhibitory\n effects of DEX on T3 secretion and T4 to T3 conversion. The tested dl- and ndl-PCBs and their hydroxylated metabolites affected the basal and DEX-modified mRNA expression and the protein concentration of all three deiodinases. The PCBs and OH-PCBs did not change\n the MCT8 gene expression; however, PCB118 and 4-OH-PCB107 reduced the MCT10 mRNA levels with a concomitant increase in the basal and DEX - -stimulated LAT1 mRNA expression. PCB153 and 3-OH-PCB153 did not influence the MCT10 expres- sion, but they elevated the\n basal and reduced DEX-stimulated LAT1 mRNA levels. Among the four tested PCBs, only 4-OH-PCB decreased the TRβ0 mRNA expression. In conclusion, to our knowledge, these results revealed for the first time that both dl-PCB and ndl-PCB and their OH-PCBs affect T3\n secretion and T4 to T3 conversion, as well as the expression of iodothyronine deiodinases and TH transporters in chicken liver. These results indicate that not only the parental PCBs, but also their hydroxylated deriva - tives may influence iodothyronine metabolism in\n a chicken's liver, resulting in changes in T3 availability in the organism.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":"1 1","pages":""},"PeriodicalIF":0.8000,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Folia Biologica-Krakow","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3409/fb_71-2.07","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

To assess the in vitro effect of polychlorinated biphenyls (PCBs) and their hydroxylated metabolites (OH - -PCBs) on the metabolism of thyroid hormones (THs) in chicken liver, explants of liver tissue were incubated in a medium supplemented with dexamethasone (DEX) (100 nM), PCB118 (dioxin-like PCB), PCB153 (non-dioxin-like PCB), 4-OH-PCB107 and 3-OH-PCB153 (0.5 × 10-8 M), and with DEX together with each of the PCBs and OH-PCBs to determine the triiodothyronine (T3) secretion, thyroxine (T4) to T3 conversion, mRNA expression and protein concentration of the iodothyronine deiodinases (DIO1, DIO2, DIO3), TH transporters (OATP1C1, MCT8, MCT10, LAT1) and TH receptors (THRA, THRB). The results obtained revealed that the tested PCBs and OH-PCBs interacted with and/or abolished the inhibitory effects of DEX on T3 secretion and T4 to T3 conversion. The tested dl- and ndl-PCBs and their hydroxylated metabolites affected the basal and DEX-modified mRNA expression and the protein concentration of all three deiodinases. The PCBs and OH-PCBs did not change the MCT8 gene expression; however, PCB118 and 4-OH-PCB107 reduced the MCT10 mRNA levels with a concomitant increase in the basal and DEX - -stimulated LAT1 mRNA expression. PCB153 and 3-OH-PCB153 did not influence the MCT10 expres- sion, but they elevated the basal and reduced DEX-stimulated LAT1 mRNA levels. Among the four tested PCBs, only 4-OH-PCB decreased the TRβ0 mRNA expression. In conclusion, to our knowledge, these results revealed for the first time that both dl-PCB and ndl-PCB and their OH-PCBs affect T3 secretion and T4 to T3 conversion, as well as the expression of iodothyronine deiodinases and TH transporters in chicken liver. These results indicate that not only the parental PCBs, but also their hydroxylated deriva - tives may influence iodothyronine metabolism in a chicken's liver, resulting in changes in T3 availability in the organism.

查看原文本刊更多论文

多氯联苯和oh -多氯联苯对鸡肝脏基础和地塞米松修饰甲状腺激素代谢的体外影响

为了评估多氯联苯（PCB）及其羟基化代谢产物（OH-PCB）对鸡肝脏甲状腺激素（THs）代谢的体外影响，将肝组织外植体在添加地塞米松（DEX）（100nM）、PCB118（二恶英样PCB）、PCB1 53（非二恶英类PCB）、4-OH-PCB107和3-OH-PCB153（0.5×10-8M）的培养基中孵育，并与DEX以及每个PCB和OH PCB一起测定三碘甲腺原氨酸（T3）的分泌、甲状腺素（T4）到T3的转化、碘甲腺氨酸脱碘酶（DIO1、DIO2、DIO3）、TH转运蛋白（OATP1C1、MCT8、MCT10、LAT1）和TH受体（THRA、THRB）的mRNA表达和蛋白浓度。所获得的结果显示，所测试的PCB和OH PCB与DEX相互作用和/或消除了DEX对T3分泌和T4向T3转化的抑制作用。测试的dl-和ndl-PCBs及其羟基化代谢产物影响所有三种脱碘酶的基础和DEX修饰的mRNA表达以及蛋白质浓度。多氯联苯和羟基多氯联苯对MCT8基因表达没有改变；然而，PCB118和4-OH-PCB107降低了MCT10 mRNA水平，同时增加了基础和DEX刺激的LAT1 mRNA表达。PCB153和3-OH-PCB153不影响MCT10的表达，但它们提高了基础水平并降低了DEX刺激的LAT1 mRNA水平。在四种测试的多氯联苯中，只有4-OH-PCB降低了TRβ0 mRNA的表达。总之，据我们所知，这些结果首次揭示了dl-PCB和ndl-PCB及其OH-PCB都会影响鸡肝脏中T3的分泌和T4到T3的转化，以及碘甲状腺原氨酸脱碘酶和TH转运蛋白的表达。这些结果表明，不仅亲代多氯联苯，而且它们的羟基化衍生物都可能影响鸡肝脏中碘甲状腺原氨酸的代谢，导致生物体中T3的可用性发生变化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Folia Biologica-Krakow 医学-生物学

CiteScore

1.10

自引率

14.30%

发文量

审稿时长

>12 weeks

期刊介绍： Folia Biologica (Kraków) is an international online open access journal accepting original scientific articles on various aspects of zoology: phylogeny, genetics, chromosomal studies, ecology, biogeography, experimental zoology and ultrastructural studies. The language of publication is English, articles are assembled in four issues per year.