Exploration of potential circulating micro-RNA as biomarker for Alzheimer's disease

IF 0.8 Q4 GENETICS & HEREDITY

Meta Gene Pub Date : 2021-12-01 DOI:10.1016/j.mgene.2021.100968

Mahsa Abolghasemi , Elham Poursaei , Soghra Bornehdeli , Dariush Shanehbandi , Milad Asadi , Mahsa Sadeghzadeh , Reza Naghdi Sadeh

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引用次数: 1

Abstract

Alzheimer's disease (AD) is considered a progressive and devastating neurodegenerative disorder which primarily effects elders. Due to the course of the disease and the involvement of different gene loci with complex genetic studies, it is possible to calculate the relative risk of individuals at risk. In this study, our aim was to investigate the serum transcript levels of candidate microRNAs (miRNAs), including miR-138, miR-128, and miR-107 in patients with AD and compared with that of normal individuals. In this research work, during the diagnosis process of AD, 50 blood samples were obtained from patients and 50 samples were collected from healthy individuals. Then total RNA of each sample was extracted and the expression levels of miRNAs were measured by Real-time PCR method. Our analysis indicated that the relative expression of all three miRNAs decreased in AD patients compared to the healthy samples. There was statistically significant in transcript levels of miR-138 (P = 0.047) and miR-107 (P = 0.004), while the levels of miR-128 had no significant difference between two groups (P = 0.075). The receiver operating characteristic (ROC) curve analysis indicated the potential of all miRNAs as biomarkers in AD. In conclusion, miR-138 and miR-107 can be used as a diagnostic or predictive biomarker in AD. In the case of miR-128, more research is needed. We suggest further studies and evaluation of changes in the expression of these miRNAs in larger populations.

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探索潜在的循环微rna作为阿尔茨海默病的生物标志物

阿尔茨海默病(AD)被认为是一种进行性和破坏性的神经退行性疾病，主要影响老年人。由于疾病的病程和涉及不同基因位点的复杂遗传研究，有可能计算出处于危险中的个体的相对风险。在这项研究中，我们的目的是研究AD患者血清中候选microRNAs (miRNAs)的转录水平，包括miR-138、miR-128和miR-107，并与正常人进行比较。在本研究工作中，在AD的诊断过程中，从患者身上采集了50份血样，从健康个体身上采集了50份血样。然后提取各样品的总RNA，采用Real-time PCR法检测mirna的表达水平。我们的分析表明，与健康样本相比，AD患者中所有三种mirna的相对表达量都有所下降。miR-138、miR-107转录本水平差异有统计学意义(P = 0.047)， miR-128转录本水平差异无统计学意义(P = 0.075)。受试者工作特征(ROC)曲线分析显示了所有mirna作为AD生物标志物的潜力。总之，miR-138和miR-107可以作为AD的诊断或预测生物标志物。对于miR-128，还需要更多的研究。我们建议进一步研究和评估这些mirna在更大人群中的表达变化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Meta Gene Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： Meta Gene publishes meta-analysis, polymorphism and population study papers that are relevant to both human and non-human species. Examples include but are not limited to: (Relevant to human specimens): 1Meta-Analysis Papers - statistical reviews of the published literature of human genetic variation (typically linked to medical conditionals and/or congenital diseases) 2Genome Wide Association Studies (GWAS) - examination of large patient cohorts to identify common genetic factors that influence health and disease 3Human Genetics Papers - original studies describing new data on genetic variation in smaller patient populations 4Genetic Case Reports - short communications describing novel and in formative genetic mutations or chromosomal aberrations (e.g., probands) in very small demographic groups (e.g., family or unique ethnic group). (Relevant to non-human specimens): 1Small Genome Papers - Analysis of genetic variation in organelle genomes (e.g., mitochondrial DNA) 2Microbiota Papers - Analysis of microbiological variation through analysis of DNA sequencing in different biological environments 3Ecological Diversity Papers - Geographical distribution of genetic diversity of zoological or botanical species.