Extraction, separation, and evaluation of antioxidant effect of the different fractions of polyphenols from cocoa beans

IF 0.2 Q4 CHEMISTRY, MULTIDISCIPLINARY

Revista Colombiana de Quimica Pub Date : 2020-11-06 DOI:10.15446/rcq.v49n3.84082

Sergio M. Pico-Hernández, Cristian J. Murillo-Méndez, L. López-Giraldo

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引用次数: 5

Abstract

Polyphenol Extracts (PE) hold antioxidant properties, which might be related to positive effects on human health. It has been stated that PE, obtained from cocoa beans, contain fractions of flavan-3-ols with different degrees of polymerization (DP). However, it is unknown which of the fractions or their mixture drives the best antioxidant activity. This paper reports the study conducted to elucidate the role of each fraction (with different DP) on Antioxidant Capacity (AC). First, the process of extraction and separation of polyphenols’ fractions in cocoa beans was executed; afterward, AC was determined for each fraction individually and their combinations (monomers, dimers and oligomers). Solid-liquid extraction was made by using a 50% (v/v) ethanol solution and a mass:solvent ratio of 1:120 in an ultrasound bath. PE were separated into monomers, dimers, and oligomers by HPLC using a semipreparative column. The results obtained show that PE contain 95.35, 7.45, and 21.75 mg EE (epicatechin equivalents) / g ds of monomers, dimers, and oligomers, respectively. Finally, the AC of each fraction was evaluated using a complete cubic model mix design. According to the results, the best AC was obtained for dimers. However, when monomers, dimers and oligomers were mixed, an antagonistic effect on AC was observed.

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可可豆中不同组分多酚的提取、分离及抗氧化效果评价

多酚提取物（PE）具有抗氧化特性，这可能与对人类健康的积极影响有关。已经指出，从可可豆中获得的PE含有具有不同聚合度（DP）的黄烷-3-醇级分。然而，尚不清楚哪种组分或其混合物具有最佳的抗氧化活性。本文报道了为阐明每种组分（具有不同DP）对抗氧化能力（AC）的作用而进行的研究。首先，对可可豆中多酚组分的提取和分离过程进行了研究；然后，分别测定每个馏分及其组合（单体、二聚体和低聚物）的AC。在超声浴中使用50%（v/v）乙醇溶液和质量∶溶剂比为1:120进行固液萃取。使用半制备柱通过HPLC将PE分离为单体、二聚体和低聚物。所获得的结果表明，PE分别含有95.35、7.45和21.75mg EE（表儿茶素当量）/g ds的单体、二聚体和低聚物。最后，使用完整的三次模型配合比设计来评估每个馏分的AC。结果表明，二聚体得到了最佳的AC。然而，当单体、二聚体和低聚物混合时，观察到对AC的拮抗作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Revista Colombiana de Quimica CHEMISTRY, MULTIDISCIPLINARY-

CiteScore

1.50

自引率

0.00%

发文量

期刊介绍： The Revista Colombiana de Química - Colombian Journal of Chemistry (Rev. Colomb. Quim.) Is a peer-reviewed scientific journal of the Department of Chemistry, Faculty of Sciences of the Universidad Nacional de Colombia, Bogotá. It currently publishes three volumes per year: January-April, May-August and September-December. All the content published by the Journal (available online) is under a Creative Commons attribution license type BY 4.0, that allows any person or entity in the world to freely access the content, share it, download it, adapt it or make derivative works without any restriction, provided that it adequately indicates the credit to the authors and to the Colombian Journal of Chemistry.