Antihyperlipidemic and Antioxidant Effects of Ethanol Fraction of Sargassum angustifolium in Dexamethasone-Induced Dyslipidemic Rats

IF 1.1 Q4 PHARMACOLOGY & PHARMACY
A. Yegdaneh, L. Safaeian, Mahnaz Halvaei-Varnousfaderani, Saeed Bazvand
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引用次数: 2

Abstract

Background and objectives: Recent data propose the beneficial antihyperlipidemic effects of several marine brown alga belonging to the genus Sargassum. In the current study, the effects of ethanol fraction of Sargassum angustifolium were assessed on dexamethasone-induced dyslipidemia in rats. Methods: The fraction was prepared by maceration method and then using a reverse phase column chromatography. It was evaluated for total phenolic and salt contents. Seven groups of six male rats were used as the following: group 1 (normal control) received vehicle for 1 week; group 2 (Sargassum control) was treated only with 80 mg/kg S. angustifolium for one week; group 3 (dyslipidemic control) received dexamethasone (10 mg/kg/day, subcutaneously) for one week; groups 4-6 (test groups) received dexamethasone and were simultaneously treated orally with 20, 40 or 80 mg/kg S. angustifolium and group 7 (reference) received dexamethasone and atorvastatin (40 mg/kg, orally) for one week. At the end of experiment, fasting blood glucose, lipid markers and malondialdehyde levels were evaluated in serum specimens. Livers were weighed and processed for histopathological inspection. Results: The content of total phenolics was 87.21 ± 2.4 mg/g as gallic acid equivalent and salt as NaCl was 6.5 g/100 g. Treatment with S. angustifolium significantly decreased serum blood sugar, triglycerides, total cholesterol, low‑density lipoprotein-cholesterol and malondialdehyde levels and also alleviated steatotic changes in liver tissues compared to the dexamethasone-induced dyslipidemic control group. Conclusion: Findings of the current study revealed anti-hyperglycemic, hypolipidemic and anti-lipid proxidative properties of S. angustifolium ethanol fraction in an animal model of dyslipidemia.
马尾草乙醇组分对地塞米松诱导的血脂异常大鼠的降血脂和抗氧化作用
背景和目的:最近的数据表明,马尾藻属的几种海洋褐藻具有有益的抗高血压作用。在本研究中,评估了马尾藻乙醇部分对地塞米松诱导的大鼠血脂异常的影响。方法:采用浸渍法和反相柱色谱法制备该级分。对其总酚和盐含量进行了评估。雄性大鼠分为7组,每组6只,如下:第1组(正常对照组)接受赋形剂治疗1周;第2组(马尾藻对照组)仅用80 mg/kg狭叶藻处理1周;第3组(血脂异常对照组)给予地塞米松(10mg/kg/天,皮下)1周;第4-6组(试验组)接受地塞米松并同时口服20、40或80mg/kg狭叶藻,第7组(参考组)接受皮质类固醇和阿托伐他汀(40mg/kg,口服)一周。实验结束时,对血清标本中的空腹血糖、脂质标志物和丙二醛水平进行评估。对肝脏进行称重和处理以进行组织病理学检查。结果:总酚含量为87.21 ± 与地塞米松诱导的血脂异常对照组相比,以没食子酸当量计为2.4 mg/g,以NaCl计为盐为6.5 g/100 g。用狭叶藻治疗可显著降低血糖、甘油三酯、总胆固醇、低密度脂蛋白胆固醇和丙二醛水平,并减轻肝组织中的脂肪变性变化。结论:目前的研究结果揭示了在血脂异常动物模型中,沙枣乙醇组分的抗高血糖、降血脂和降脂作用。
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来源期刊
Research Journal of Pharmacognosy
Research Journal of Pharmacognosy PHARMACOLOGY & PHARMACY-
CiteScore
1.10
自引率
20.00%
发文量
0
审稿时长
8 weeks
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