Apigenin targets protein and lipids of HeLa cells and exhibits protective efficacy against oxidative stress

IF 0.4 4区 医学 Q4 PHARMACOLOGY & PHARMACY
B. Pawlikowska-Pawlęga, J. Kapral-Piotrowska, A. Jarosz-Wilkołazka, B. Chudzik, R. Paduch, J. Jakubowicz-Gil, A. Wawrzyniak, I. Łuszczewska-Sierakowska, A. Sławińska-Brych, B. Zdzisińska, E. Reszczyńska, K. Trebacz, W. Gruszecki
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Abstract

Apigenin, non-toxic and non-mutagenic flavonoid, is an alternative to classical drugs that interact with membranes. Hence, the objects of the study were to examine its effects on liposomes made of egg yolk phosphatidylcholine (EYPC) with the use of 1H NMR, four-electrode BLM and EPR techniques, and determine its activity on lipids and proteins of human cervix carcinoma (HeLa) cells. In addition its protective efficacy against H2O2- induced oxidative shock was investigated. FTIR spectroscopy was applied to study molecular interactions with membrane lipids and proteins of HeLa cells. Microscopic techniques (SEM, light, and fluorescence), flow cytometer analysis, and NR assays were employed to reveal apigenin involvement in apoptosis in different cell conditions. Apigenin affected mainly the region of choline head groups and the hydrophobic core below this area. Simultaneously, the ordering effect was shown. The fingerprint region of lipids in the HeLa cells was found as a target for apigenin. Furthermore, in the amide I and amide II regions, a decrease in β-sheets and an increase in turns, loops, and unordered structures were noted. Apigenin reduced viability of cells and induced apoptosis. SEM observations revealed characteristic changes in morphology of the examined cells. Pretreatment of HeLa cells with apigenin protected them against H2O2-induced oxidative stress by the increase in glutathione content as well as superoxide dismutase and catalase levels. The consequences of molecular changes related to membranes and cancer cells make apigenin a unique and very interesting bio-compound with great significance for medical and biological applications.
芹菜素靶向HeLa细胞的蛋白质和脂质,对氧化应激具有保护作用
芹菜素是一种无毒、非诱变的类黄酮,是与膜相互作用的经典药物的替代品。因此,本研究的目的是利用1H NMR、四电极BLM和EPR技术研究其对蛋黄磷脂酰胆碱(EYPC)脂质体的影响,并测定其对人宫颈癌(HeLa)细胞脂质和蛋白质的活性。此外,还研究了其对H2O2诱导的氧化休克的保护作用。利用FTIR光谱研究了HeLa细胞与膜脂和蛋白的相互作用。显微技术(扫描电镜、光和荧光)、流式细胞仪分析和NR分析揭示了芹菜素在不同细胞条件下参与凋亡的作用。芹菜素主要影响胆碱头基团区域及其以下疏水核。同时,还显示了排序效应。在HeLa细胞中发现脂质指纹区是芹菜素的靶点。此外,在酰胺I和酰胺II区,β-片减少,匝、环和无序结构增加。芹菜素降低细胞活力,诱导细胞凋亡。扫描电镜观察显示所检查细胞的形态学特征变化。芹菜素预处理HeLa细胞可通过提高谷胱甘肽含量、超氧化物歧化酶和过氧化氢酶水平,保护HeLa细胞免受h2o2诱导的氧化应激。与细胞膜和癌细胞相关的分子变化的后果使芹菜素成为一种独特而非常有趣的生物化合物,具有重要的医学和生物学应用意义。
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来源期刊
CiteScore
0.80
自引率
0.00%
发文量
74
审稿时长
6-12 weeks
期刊介绍: The international journal of the Polish Pharmaceutical Society is published in 6 issues a year. The journal offers Open Access publication of original research papers, short communications and reviews written in English, in all areas of pharmaceutical sciences. The following areas of pharmaceutical sciences are covered: Analysis, Biopharmacy, Drug Biochemistry, Drug Synthesis, Natural Drugs, Pharmaceutical Technology, Pharmacology and General. A bimonthly appearing in English since 1994, which continues “Acta Poloniae Pharmaceutica”, whose first issue appeared in December 1937. The war halted the activity of the journal’s creators. Issuance of “Acta Poloniae Pharmaceutica” was resumed in 1947. From 1947 the journal appeared irregularly, initially as a quarterly, then a bimonthly. In the years 1963 – 1973 alongside the Polish version appeared the English edition of the journal. Starting from 1974 only works in English are published in the journal. Since 1995 the journal has been appearing very regularly in two-month intervals (six books a year). The journal publishes original works from all fields of pharmacy, summaries of postdoctoral dissertations and laboratory notes.
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