Photosynthesis-related responses of Chlorella sorokiniana (Trebouxiophyceae) to environmentally relevant concentrations of Cu nanoparticles

IF 1.5 4区生物学 Q3 MARINE & FRESHWATER BIOLOGY

Phycologia Pub Date : 2023-06-16 DOI:10.1080/00318884.2023.2214777

D. Barreto, A. Tonietto, Everton Willian Rodrigues da Silva Oliveira, Clarice Dias Britto Amaral, R. C. Pulgrossi, A. Polpo, J. Nóbrega, A. T. Lombardi

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Abstract

ABSTRACT Nanoparticles (NPs) are highly reactive particles that find a broad array of applications in society; as a result, they may accumulate in aquatic environments. Microalgae are the base of foodwebs and serve as surfaces for NPs, interacting and transporting them to higher trophic levels. We aimed at understanding the effects of environmentally significant and higher copper nanoparticles (Cu-NPs) concentrations on the photosynthetic performance of Chlorella sorokiniana, which included determining effective quantum yield (F’v/F’m), photochemical (Qp) and non-photochemical (NPQ) quenching, rapid light curves (RLC), light use efficiency (α), minimum saturation irradiance (Ek) and the maximum relative electron transport rate (ETRrmax), in addition to chlorophyll a content and cell viability. Dissolved Cu and free ionic Cu concentrations were determined, and dissolved Cu values were used as surrogate for nominal Cu-NPs concentrations. The experiments lasted 72 h and were carried out under controlled conditions. The results showed that cell viability and chlorophyll a decreased as Cu-NPs increased, but the opposite was obtained for the RLC parameters. Light saturation (Ek) and ETR increased as Cu-NPs concentrations increased in culture medium, but not the efficiency with which C. sorokiniana used the light. By showing that environmentally relevant Cu-NPs influenced C. sorokiniana metabolism, we add to the knowledge on the interactions between environmentally realistic Cu-NPs levels and phytoplankton cells.

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Sorokiana小球藻（Trebouxiphyceae）对环境相关浓度的Cu纳米颗粒的光合作用相关反应

摘要纳米颗粒是一种高活性颗粒，在社会上有着广泛的应用；因此，它们可能会在水生环境中积累。微藻是食物网的基础，是NP的表面，相互作用并将其输送到更高的营养水平。我们旨在了解具有环境意义的更高浓度的铜纳米颗粒（Cu-NPs）对索氏小球藻光合性能的影响，包括确定有效量子产率（F'v/F'm）、光化学（Qp）和非光化学（NPQ）猝灭、快速光曲线（RLC）、光利用效率（α），最小饱和辐照度（Ek）和最大相对电子传输速率（ETRmax）。测定溶解的Cu和游离离子Cu浓度，并使用溶解的Cu值作为标称Cu NP浓度的替代品。实验持续72小时，并在受控条件下进行。结果表明，随着Cu NPs的增加，细胞活力和叶绿素a降低，但RLC参数则相反。光饱和（Ek）和ETR随着培养基中Cu NPs浓度的增加而增加，但不影响索氏藻利用光的效率。通过表明与环境相关的Cu NPs影响了Sorokiana C.的代谢，我们增加了关于环境现实Cu NPs水平与浮游植物细胞之间相互作用的知识。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Phycologia 生物-海洋与淡水生物学

CiteScore

3.10

自引率

18.80%

发文量

审稿时长

3 months

期刊介绍： Phycologia is published bimonthly by the International Phycological Society and serves as a publishing medium for information about any aspect of phycology. Membership in the Society is not necessary for publication. Submitted manuscripts cannot be previously published or submitted elsewhere. Copyright ownership of all accepted papers is held by the International Phycological Society.