Screening of Stb leaf blotch resistance genes of the common wheat varieties

N. Novoselova, A. Kharina, E. A. Bessolitsina, L. S. Savintseva
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Abstract

Currently, leaf blotch is one of the main problems in wheat production around the world. The use of DNA markers for screening a large amount of breeding material makes it possible to identify wheat leaf blotch resistance genes quickly and accurately. In this regard, the Federal Agricultural Research Center of the North-East named after N. V. Rudnitsky conducted a search for DNA markers linked to Z. tritici resistance in the genotypes of the collection material of common wheat. There were studied 75 spring and 12 winter bread wheat varieties for the presence of the Stb2, Stb11, Stb12 and Stb13 genes. As a result, Stb leaf blotch resistance genes were found only in 8.0 % of varieties. In the genotype of the spring wheat variety ‘Irmenka 1’, there was determined the presence of three loci of resistance Stb2, Stb11 and Stb13, which were responsible for age-related and juvenile resistance to the disease. The genes of juvenile leaf blotch resistance Stb11 and Stb13 were found in the genotype of the spring common wheat variety ‘Tertsiya’. The Stb2 gene controlling age-related leaf blotch resistance was found in the genotypes of spring wheat varieties ‘Dariya’, ‘Egisar 29’ and ‘Epos’. In the genotypes of winter varieties ‘Gene’ and ‘Ble-seigle’, there were found one gene Stb11 and Stb13, respectively. The Stb12 leaf blotch resistance gene was not identified in the studied wheat varieties. All spring and winter common wheat varieties with Stb-genes can be recommended as sources of leaf blotch resistance in breeding with highly productive varieties adapted to local conditions.
普通小麦品种Stb叶斑病抗性基因的筛选
目前,叶斑病是世界各地小麦生产中的主要问题之一。利用DNA标记对大量育种材料进行筛选,可以快速准确地鉴定小麦抗叶斑病基因。在这方面,以N.V.Rudnitsky命名的东北部联邦农业研究中心在普通小麦采集材料的基因型中搜索了与小小麦抗性相关的DNA标记。对75个春小麦和12个冬小麦品种的Stb2、Stb11、Stb12和Stb13基因进行了研究。结果,仅在8.0%的品种中发现了Stb叶斑病抗性基因。在春小麦品种“Irmenka 1”的基因型中,确定了三个抗性位点Stb2、Stb11和Stb13的存在,这三个位点与年龄相关和幼年期对该疾病的抗性有关。在春小麦品种“Tertsiya”的基因型中发现了幼叶斑点病抗性基因Stb11和Stb13。在春小麦品种“Dariya”、“Egisar 29”和“Epos”的基因型中发现了控制年龄相关性叶斑病抗性的Stb2基因。在冬季品种‘Gene’和‘Ble-seigle’的基因型中,分别发现一个基因Stb11和Stb13。在所研究的小麦品种中没有鉴定出Stb12叶斑病抗性基因。所有具有Stb基因的春冬季普通小麦品种都可以作为抗叶斑病的来源,用于选育适合当地条件的高产品种。
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