Molecular Detection of Toxoplasma gondii in Chicken Meats and Eggs in Semnan City, Iran

Q4 Veterinary
F. Mehrabi, M. Rassouli, S. H. Emadi Chashmi
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引用次数: 0

Abstract

ackground: Toxoplasma gondii is a protozoan parasite, a member of the phylum Apicomplexa. Felids are definitive hosts, and all warm-blooded animals and humans are intermediate hosts. The clinical symptoms of toxoplasmosis among chickens are mostly subclinical, but the infection of chickens and eggs is important as a source of protein for human consumption. Objectives: This study aimed to detect T. gondii in chicken meat and egg by molecular examination. Methods: In this study, 100 chicken legs, 50 eggs of free-range hens, and 50 eggs of industrial hens were collected from different stores in Semnan City, Iran. The samples were inspected for the Toxoplasma B1 gene after DNA extraction. Results: According to the results, Toxoplasma DNA was detected in 23% of chicken legs, 36% of eggs of free-range hens, and 20% of eggs of industrial hens. The infection rate was not significantly different between eggs of free-range and industrial hens (P>0.05). Conclusion: Therefore, Toxoplasma is present in chicken meats and eggs in Semnan, Iran, and it is recommended that people eat well-cooked chicken meat and eggs for disease control and feed domestic carnivores with cooked meat to prevent the parasite life cycle.
伊朗塞姆南市鸡肉和鸡蛋中弓形虫的分子检测
背景:弓形虫是一种原生动物寄生虫,属于Apicompleta门。猫科动物是最终宿主,所有温血动物和人类都是中间宿主。鸡弓形虫病的临床症状大多为亚临床症状,但鸡和鸡蛋的感染是人类食用蛋白质的重要来源。目的:采用分子检测方法检测鸡肉和鸡蛋中的弓形虫。方法:在本研究中,从伊朗塞姆南市的不同商店收集了100只鸡腿、50只散养母鸡的蛋和50只工业母鸡的蛋。在DNA提取后对样品进行弓形虫B1基因检查。结果:弓形虫DNA在鸡腿中检出率为23%,在散养母鸡鸡蛋中检出率36%,在工业母鸡鸡蛋中检测率为20%。结论:在伊朗塞姆南,鸡的肉和蛋中均存在弓形虫,建议人们食用煮熟的鸡肉和蛋来控制疾病,并用煮熟的肉喂养国内食肉动物以防止寄生虫的生命周期。
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来源期刊
Iranian Journal of Veterinary Medicine
Iranian Journal of Veterinary Medicine Veterinary-General Veterinary
CiteScore
0.90
自引率
0.00%
发文量
0
审稿时长
6 weeks
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