Hippocampal electrophysiological responses and changes in oxidative stress marker and serum lipid profile to pharmacological and non-pharmacological treatments of high-fat-fructose diet induced metabolic syndrome

European Pharmaceutical Journal Pub Date : 2019-06-01 DOI:10.2478/afpuc-2019-0007

D. Michalikova, B. Kaprinay, M. Sasváriová, K. Švík, L. Slovák, R. Sotníková, T. Stankovičová, Z. Gasparova

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引用次数: 1

Abstract

Abstract The aim of our study was to evaluate the possibility of influencing the risk factors of metabolic syndrome (MetS) and metabolic cognitive syndrome. As a model of MetS, we used high-fat-fructose diet (HFFD) fed hypertriacylglycerolemic (HTG) rats. Control group included HTG rats fed with HFFD during 8 weeks (HFFD8). Furthermore, we tested the effect of pharmacological and non-pharmacological therapies. Non-pharmacological therapy, which we chose, was a change in diet from HFFD (5 weeks) to standard one (3 weeks) and thus caloric restriction (HFFD5+3). The drug we used was rosmarinic acid (RA; 100mg/kg), which we administered to rats after 5 weeks of HFFD once a day for consecutive 3 weeks with current change in diet to standard one (HFFD5+3+RA) or during lasting last 3 weeks of HFFD (HFFD8+RA). After 8 weeks of experiment, lipid peroxidation markers, lipid profile of blood serum, and neuronal transmission and synaptic plasticity (long-term potentiation [LTP]) in hippocampal sections were evaluated in vitro. We observed a significant effect of dietary change in lipid profile (decreased total cholesterol and low-density lipoprotein cholesterol [LDL-cholesterol] and increased high-density lipoprotein cholesterol [HDL-cholesterol]). The combination of pharmacological and non-pharmacological treatments caused a decrease in total cholesterol, LDL-cholesterol, and lipid peroxidation in blood serum. Change in HFFD to standard diet without treatment resulted in slight improvement in neuronal transmission in the hippocampus and caloric restriction alone also had positive effect on LTP maintenance. Our results suggest that combination of pharmacological and non-pharmacological approaches had better impact on the biochemical parameters of MetS in blood serum, but weak impact on neuronal functions in the hippocampus, where the expected positive effect was achieved only by caloric restriction.

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高脂果糖饮食诱导代谢综合征的海马电生理反应及氧化应激标志物和血脂谱的变化

摘要本研究的目的是评估影响代谢综合征（MetS）和代谢认知综合征危险因素的可能性。作为代谢综合征的模型，我们使用高脂肪果糖饮食（HFFD）喂养的高甘油三酯血症（HTG）大鼠。对照组包括8周内喂食HFFD的HTG大鼠（HFFD8）。此外，我们还测试了药物和非药物疗法的效果。我们选择的非药物治疗是将饮食从HFFD（5周）改为标准饮食（3周），从而限制热量（HFFD5+3）。我们使用的药物是迷迭香酸（RA；100mg/kg），在HFFD 5周后，我们每天给大鼠服用一次，连续3周，目前饮食改为标准饮食（HFFD5+3+RA）或在HFFD持续最后3周期间（HFFD8+RA）。实验8周后，在体外评估海马切片中的脂质过氧化标记物、血清脂质图谱、神经元传递和突触可塑性（长时程增强[LTP]）。我们观察到饮食变化对脂质状况的显著影响（总胆固醇和低密度脂蛋白胆固醇[LDL胆固醇]降低，高密度脂蛋白蛋白胆固醇[HDL胆固醇]增加）。药物和非药物治疗相结合可降低血清中的总胆固醇、低密度脂蛋白胆固醇和脂质过氧化。将HFFD改变为不治疗的标准饮食导致海马中神经元传递的轻微改善，单独的热量限制也对LTP维持有积极影响。我们的研究结果表明，药理学和非药理学方法的结合对血清中MetS的生化参数有更好的影响，但对海马神经元功能的影响较弱，只有通过热量限制才能达到预期的积极作用。

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来源期刊

European Pharmaceutical Journal Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

0.60

自引率

0.00%

发文量

期刊介绍： European Pharmaceutical Journal publishes only original articles not previously published and articles that are not being considered or have not been submitted for publication elsewhere. If parts of the results have been published as conference abstract or elsewhere, it should be stated in references. The ethical standards of the Helsinki-Tokio Declaration should be kept. This should be mentioned in the Methods of manuscript. Reviews are published only on request. Authors, whose submitted research work was performed with the support of a company, should indicate this in Conflict of Interest.