Acoustic Levitation assisted Contactless Printing of Microdroplets for Biomedical Applications

IF 2.4 3区 工程技术 Q3 ENGINEERING, MANUFACTURING
Tengteng Tang, Dylan Joralmon, Tochukwu Anyigbo, Xiangjia Li
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引用次数: 0

Abstract

The artificial cell is a biomimetic microcapsule system wherein biological materials are encapsulated by a thin membrane, which provides valuable information on the metabolism, morphology, development, and signal transduction pathways of the studied cell. However, it is extremely difficult to manufacture such systems. Mostly vesicles such as liposomes, polymersomes, and microcapsules are first produced by a high-pressure homogenizer and microfluidizer as an emulsion and then encapsulated microcapsules by the drop or emulsion method. Currently, acoustic levitation opens up entirely new possibilities for creating artificial cells because of its ability to suspend tiny droplets in an anti-gravity and non-contact manner. Herein, we propose a contactless printing of single-core or multi-core artificial cells based on acoustic levitation. First, the oscillation mode and microscopic morphology of the droplets under different ultrasonic vibration frequencies are shown by simulation, and the curing characteristics of the shell structure under different ultraviolet illumination conditions are quantitatively measured. The feasibility of manufacturing multi-core artificial cells and manufacturing sub-millimeter-scale particles based on oil trapping is extensively studied. To explore the morphological adaptability of artificial cells, ferromagnetic Fe3O4 nanoparticles are used to give cells magnetic responsive properties and the microscopic deformation and motion in microfluidic channels under the magnetic field are characterized. Finally, the proposed printing method proves the versatility of in-space contactless printing of complex 3D beam structures and provides a powerful platform for developing biomedical devices and microrobots and studying morphogenesis and synthetic biological systems
声学悬浮辅助生物医学应用微液滴非接触式打印
人工细胞是一种仿生微胶囊系统,其中生物材料被薄膜包裹,这为所研究细胞的代谢、形态、发育和信号转导途径提供了有价值的信息。然而,制造这样的系统是极其困难的。大多数囊泡,如脂质体、多聚体和微胶囊,首先通过高压均化器和微流器作为乳液生产,然后通过滴注或乳液法封装微胶囊。目前,声学悬浮为创造人造细胞开辟了全新的可能性,因为它能够以反重力和非接触的方式悬浮微小液滴。在此,我们提出了一种基于声学悬浮的单核或多核人工细胞的非接触式打印。首先,通过模拟显示了液滴在不同超声振动频率下的振荡模式和微观形态,并定量测量了壳结构在不同紫外线照射条件下的固化特性。广泛研究了基于油捕集制造多核人工细胞和亚毫米级颗粒的可行性。为了探索人工细胞的形态适应性,使用铁磁性Fe3O4纳米粒子赋予细胞磁响应特性,并表征了磁场下微流体通道中的微观变形和运动。最后,所提出的打印方法证明了复杂三维梁结构的空间非接触式打印的通用性,并为开发生物医学设备和微型机器人以及研究形态发生和合成生物系统提供了强大的平台
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来源期刊
CiteScore
6.80
自引率
20.00%
发文量
126
审稿时长
12 months
期刊介绍: Areas of interest including, but not limited to: Additive manufacturing; Advanced materials and processing; Assembly; Biomedical manufacturing; Bulk deformation processes (e.g., extrusion, forging, wire drawing, etc.); CAD/CAM/CAE; Computer-integrated manufacturing; Control and automation; Cyber-physical systems in manufacturing; Data science-enhanced manufacturing; Design for manufacturing; Electrical and electrochemical machining; Grinding and abrasive processes; Injection molding and other polymer fabrication processes; Inspection and quality control; Laser processes; Machine tool dynamics; Machining processes; Materials handling; Metrology; Micro- and nano-machining and processing; Modeling and simulation; Nontraditional manufacturing processes; Plant engineering and maintenance; Powder processing; Precision and ultra-precision machining; Process engineering; Process planning; Production systems optimization; Rapid prototyping and solid freeform fabrication; Robotics and flexible tooling; Sensing, monitoring, and diagnostics; Sheet and tube metal forming; Sustainable manufacturing; Tribology in manufacturing; Welding and joining
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