Gelatin Endorses the Pluripotent Stem Cells from the Reprogrammed Fibroblasts of Adult Mice Differentiation into Subtypes of Neurons in the Feeder-Free Condition for a Long-Term

Abstract

Stem cell research and application in the basic and clinical fields have been leading a prevalent in the past 20 years since these present significantly promising meanings. The relevant technologies have momentously advanced, including the cellular matrix to offer stem cells growing healthily environment. Stem cells can originate from several resources such as embryonic and induced pluripotent stem cells (iPSCs) but they all are dependent on a monolayer of feeder-cells, which are, in the original and most time, on the primary mouse embryonic fibroblasts (MEF feeder cells) to sustain their self-renewal growing. However, even though MEF application is still widely applied in the research laboratory and commercial supplies, it meets inordinate challenging since it appears some noteworthy problems such as zoonosis, contaminations, and inconsistence outcomes. Importantly, the hindmost of those halts the downstream applications of stem cells, in particular, in the clinical application. Thus, scientists have hunted around the replacements either biomaterials or non-biomaterials and their mixtures. Recently, we magnificently employed the gelatin-alone-coated dishes in feeder-free condition with the opposite medium for the proliferation of iPSCs derived from the reprogrammed fibroblasts of the adult mouse to overcome these drawbacks of MEF. Moreover, such dishes sponsor the differentiation of iPSCs into succeeding neural progenitor cells, neurons, and as the ultimate product subtype of neurons - dopaminergic neurons up to 6 months. This system is simple, modest to accomplish with lower expenses