Effects of Avocado/Soybean on the Chondrogenesis of Human Adipose-Derived Stem Cells Cultured on Polylactic-Co-Glycolic Acid/Fibrin Hybrid Scaffold

Q3 Biochemistry, Genetics and Molecular Biology

Journal of Applied Biotechnology Reports Pub Date : 2019-12-05 DOI:10.29252/jabr.06.04.03

B. Hashemibeni, Mohammad Mardani, A. Valiani, M. Pourentezari, M. Anvari, M. Yadegari, E. Mangoli

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引用次数: 5

Abstract

Introduction: Avocado and soya unsaponifiables (ASU) have been reported to be useful for the treatment of cartilage diseases. The aim of this study was to detect whether avocado/soybean can have any effect on the chondrogenesis of human adipose-derived stem cells on polylactic-co-glycolic acid/fibrin hybrid scaffold or not. Materials and Methods: The poly-lactic-co-glycolic acid (PLGA)/fibrin scaffolds were seeded with cultured human adipose tissue-derived stem cells (hADSCs), which were then divided into three groups: control, TGF-β3, and ASU and the results were analyzed 14 days later. The viability of the cells in different groups were assessed by MTT. The expression of chondrogenic-related genes Sox9, type II collagen, Aggrecan, type X collagen, and type I collagen were quantified by real time polymerase chain reaction (PCR). Protein expression levels of collagen type II and X were evaluated by Western blotting. Results: Enhanced cellular viability was observed in the ASU group compared to the transforming growth factor beta-3 (TGF-β3) group. Analysis of aggrecan (Agg), type II collagen (Coll2) and SOX9 revealed that ASU and TGF-β3 induce hADSCs on PLGA/fibrin scaffold to differentiate into chondrocytes in-vitro. Moreover, a significant decrease was observed in the expression of type X (Coll10) and I collagen (Coll1) genes in the ASU group compared to the TGF-β3 group. Protein levels of type II collagen (Coll2) significantly increased in TGF-β3 and ASU groups in comparison with those of the control group. However, protein levels of Type X collagen (Coll10) significantly declined in the ASU group when compared with the TGF-β3 group. Conclusions: The results of the present study indicated that hADSCs containing the ASU in PLGA/fibrin hybrid scaffold are an effective way to potentially enhance Cartilage-specific genes with less hypertrophy and Fibrosis in-vitro.

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牛油果/大豆对聚乳酸-羟基乙酸/纤维蛋白复合支架培养的人脂肪干细胞成软骨的影响

引言：鳄梨和大豆不皂化物（ASU）已被报道可用于治疗软骨疾病。本研究的目的是检测鳄梨/大豆是否对聚乳酸-乙醇酸/纤维蛋白混合支架上的人类脂肪干细胞的软骨形成有任何影响。材料和方法：用培养的人脂肪组织来源干细胞（hADSCs）接种聚乳酸-乙醇酸（PLGA）/纤维蛋白支架，将其分为对照组、TGF-β3组和ASU组，14天后对结果进行分析。MTT法检测各组细胞的活力。软骨形成相关基因Sox9、II型胶原、Aggrecan、X型胶原和I型胶原的表达通过实时聚合酶链式反应（PCR）进行定量。通过蛋白质印迹评估II型胶原和X型胶原的蛋白质表达水平。结果：与转化生长因子β-3（TGF-β3）组相比，ASU组的细胞活力增强。聚集蛋白聚糖（Agg）、II型胶原（Coll2）和SOX9的分析表明，ASU和TGF-β3在体外诱导PLGA/纤维蛋白支架上的hADSCs分化为软骨细胞。此外，与TGF-β3组相比，ASU组中X型（Coll10）和I型胶原（Coll1）基因的表达显著降低。与对照组相比，TGF-β3和ASU组的II型胶原（Coll2）蛋白水平显著升高。然而，与TGF-β3组相比，ASU组的X型胶原（Coll10）蛋白水平显著下降。结论：本研究结果表明，在PLGA/纤维蛋白混合支架中含有ASU的hADSCs是一种在体外增强软骨特异性基因的有效方法，可减少肥大和纤维化。

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来源期刊

Journal of Applied Biotechnology Reports Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

1.90

自引率

0.00%

发文量

期刊介绍： The Journal of Applied Biotechnology Reports (JABR) publishes papers describing experimental work relating to all fundamental issues of biotechnology including: Cell Biology, Genetics, Microbiology, Immunology, Molecular Biology, Biochemistry, Embryology, Immunogenetics, Cell and Tissue Culture, Molecular Ecology, Genetic Engineering and Biological Engineering, Bioremediation and Biodegradation, Bioinformatics, Biotechnology Regulations, Pharmacogenomics, Gene Therapy, Plant, Animal, Microbial and Environmental Biotechnology, Nanobiotechnology, Medical Biotechnology, Biosafety, Biosecurity, Bioenergy, Biomass, Biomaterials and Biobased Chemicals and Enzymes. Journal of Applied Biotechnology Reports promotes a special emphasis on: -Improvement methods in biotechnology -Optimization process for high production in fermentor systems -Protein and enzyme engineering -Antibody engineering and monoclonal antibody -Molecular farming -Bioremediation -Immobilizing methods -biocatalysis