Paul V Gwozdz, Jann Harberts, R. Zierold, R. Blick
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引用次数: 1
Abstract
Abstract We demonstrate operation of a micropore based flow cytometer in the radio-frequency range. Apart from simply counting micron sized particles, such as cells, with close to nano-second resolution this counter offers the additional benefit of delivering insight into the intracellular environment. Such non-invasive screening of the cell’s interior based on analysing amplitude and phase of the signal is helpful in characterizing the biological activity of cells. In detail we are using heterodyne mixing to demodulate the temporal impedance changes, which are induced by cells translocating through a micropore embedded in a radio-frequency circuit. This allows us to measure every amplitude and phase modulation induced by a translocation event. Herein, we compare the Jurkat cells (human T lymphocytes) recordings with a control group of polystyrene beads. As the cells are measured on a single cell level, the variations on the measured amplitude and phase signals are used, herein, to sense morphological cell changes in real time.
期刊介绍:
Frequenz is one of the leading scientific and technological journals covering all aspects of RF-, Microwave-, and THz-Engineering. It is a peer-reviewed, bi-monthly published journal.
Frequenz was first published in 1947 with a circulation of 7000 copies, focusing on telecommunications. Today, the major objective of Frequenz is to highlight current research activities and development efforts in RF-, Microwave-, and THz-Engineering throughout a wide frequency spectrum ranging from radio via microwave up to THz frequencies.
RF-, Microwave-, and THz-Engineering is a very active area of Research & Development as well as of Applications in a wide variety of fields. It has been the key to enabling technologies responsible for phenomenal growth of satellite broadcasting, wireless communications, satellite and terrestrial mobile communications and navigation, high-speed THz communication systems. It will open up new technologies in communications, radar, remote sensing and imaging, in identification and localization as well as in sensors, e.g. for wireless industrial process and environmental monitoring as well as for biomedical sensing.