Pre-probiotic effects of different bacterial species in aquaculture: behavioral, hematological and oxidative stress responses

IF 0.9 4区环境科学与生态学 Q4 OCEANOGRAPHY

Oceanological and Hydrobiological Studies Pub Date : 2022-06-01 DOI:10.26881/oandhs-2022.2.01

G. Alak, R. Kotan, A. Uçar, Veysel Parlak, M. Atamanalp

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引用次数: 2

Abstract

Abstract There is very limited aquaculture research on candidate probiotics and their effects on fish physiology. In this study, acute applications of four different molecularly identified bacterial species – Brevibacillus brevis FD-1 (A), Pseudomonas fluorescens FDG-37 (B), Bacillus sphaericus FD-48 (C), and B. amyloliquefaciens TV-17C (D), with potential in aquaculture, were tested in rainbow trout (Oncorhynchus mykiss) under static conditions. Physiological changes in blood tissue [hematological indices: erythrocyte count (RBC), leukocyte count (WBC), hemoglobin (Hb), hematocrit (Hct), platelet count (PLT), mean cell hemoglobin concentration (MCHC), mean cell hemoglobin (MCH), and mean cell volume (MCV)], oxidative stress responses in liver and gill tissues [malondialdehyde (MDA) level, antioxidant enzyme activities: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR), glutathione-S-transferase (GST), glucose-6-phosphate dehydrogenase (G6PD)] and acetylcholinesterase (AChE) activity in brain tissue (as neurotoxic biomarker) were investigated. Additionally, behavioral differences were recorded by measuring swimming performance to support neurotoxic findings in all treatment groups. The LC5024 value of FDG-37 strain was determined through analysis as 1.0 × 108 CFU ml-1. Inhibition of enzyme activity, increase in the MDA level, as well as significant differences in hematological indices and swimming performance were determined in rainbow trout treated with B compared to control and other bacterial groups in gills. The potential for using group FD-48 and TV-17C bacterial strains as probiotics in aquaculture is more pertinent when considering the research findings and water quality parameters.

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水产养殖中不同细菌种类的益生菌前效应：行为、血液学和氧化应激反应

摘要关于候选益生菌及其对鱼类生理学影响的水产养殖研究非常有限。在本研究中，在静态条件下，在虹鳟（Oncorhynchus mykiss）中测试了四种不同分子鉴定的细菌物种——短短短杆菌FD-1（A）、荧光假单胞菌FDG-37（B）、球形芽孢杆菌FD-48（C）和解淀粉芽孢杆菌TV-17C（D）在水产养殖中的急性应用。血液组织的生理变化[血液学指标：红细胞计数（RBC）、白细胞计数（WBC）、血红蛋白（Hb）、红细胞比容（Hct）、血小板计数（PLT）、平均细胞血红蛋白浓度（MCHC）、平均血红蛋白（MCH）和平均细胞体积（MCV）]，研究了肝和鳃组织的氧化应激反应[丙二醛（MDA）水平、抗氧化酶活性：超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPX）、谷胱甘肽还原酶（GR）、谷胱甘肽-S-转移酶（GST）、葡萄糖-6-磷酸脱氢酶（G6PD）]和脑组织乙酰胆碱酯酶（AChE）活性（作为神经毒性生物标志物）。此外，通过测量游泳成绩来记录行为差异，以支持所有治疗组的神经毒性发现。经分析，FDG-37菌株的LC5024值为1.0×。与对照组和鳃中的其他细菌组相比，用B处理的虹鳟鱼的酶活性受到抑制，MDA水平增加，血液学指标和游泳性能也存在显著差异。考虑到研究结果和水质参数，在水产养殖中使用FD-48和TV-17C组菌株作为益生菌的潜力更为重要。

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来源期刊

Oceanological and Hydrobiological Studies 地学-海洋学

CiteScore

1.70

自引率

11.10%

发文量

审稿时长

>12 weeks

期刊介绍： Oceanological and Hydrobiological Studies is an international journal published by the Institute of Oceanography, University of Gdańsk in Poland. The journal has 4 issues per year and contains papers on all aspects of the marine environment and hydrobiology. All manuscripts are reviewed by editors and independent experts. Based on the referees'' recommendations, the Editor will make a decision on whether to accept a contribution. All articles are published in English. The journal is open to all matters concerning the water environment, thus providing the readers with a wide spectrum of topics in every issue.