Analysis of TGFB1, CD105 and FSP1 expression in human granulosa cells during a 7-day primary in vitro culture

Q4 Biochemistry, Genetics and Molecular Biology

Medical Journal of Cell Biology Pub Date : 2020-12-31 DOI:10.2478/acb-2020-0019

A. Bryja, W. Pieńkowski, K. Stefańska, B. Chermuła, Rut Bryl, M. Wieczorkiewicz, Jakub Kulus, G. Wąsiatycz, D. Bukowska, Kornel Ratajczak, J. Jaśkowski, J. Petitte, P. Mozdziak, L. Pawelczyk, R. Spaczyński, P. Antosik

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引用次数: 1

Abstract

Abstract The human granulosa cells (GCs) surround the oocyte and form the ovarian follicle’s proper architecture. These sub-populations include mural granulosa cells, antral granulosa cells, and cumulus granulosa cells. Their main functions are to support the oocyte’s growth (cumulus granulosa cells) and estradiol production (mural granulosa cells). After ovulation, the granulosa cells transform into the luteal cells of the corpus luteum and produce progesterone. Our study investigated the expression profile of three genes: TGFB1, CD105, and FSP1 during a 7-day in vitro culture. The analysis was conducted using the RT-qPCR technique. Changes in the expression of CD105 and FSP1 could be observed during the 7-day in vitro culture. In the case of TGFB, the expression remained at a similar level, with no statistically significant differences observed. Running title: Expression of TGFB1, CD105 and FSP1 in granulosa cells

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人颗粒细胞体外原代培养7天过程中TGFB1、CD105和FSP1表达的分析

人颗粒细胞(GCs)包裹在卵母细胞周围，形成卵巢卵泡的正常结构。这些亚群包括壁粒细胞、窦粒细胞和积云粒细胞。它们的主要功能是支持卵母细胞的生长(卵丘颗粒细胞)和雌二醇的产生(壁颗粒细胞)。排卵后，颗粒细胞转化为黄体的黄体细胞并产生黄体酮。我们的研究研究了TGFB1、CD105和FSP1三种基因在体外培养7天中的表达谱。采用RT-qPCR技术进行分析。在体外培养的7天内，可以观察到CD105和FSP1的表达变化。在TGFB的情况下，表达保持在相似的水平，没有观察到统计学上的显著差异。题目:颗粒细胞中TGFB1、CD105和FSP1的表达

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Medical Journal of Cell Biology Biochemistry, Genetics and Molecular Biology-Cell Biology

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