Effects of ethephon on serum levels of sex hormone, apoptosis, and cell cycle of ovaries in mice.

IF 2 4区医学 Q3 ENDOCRINOLOGY & METABOLISM

Endokrynologia Polska Pub Date : 2022-04-27 DOI:10.5603/EP.a2022.0025

Hongyuan Huang, Xiaoqiu Zhao, X. Shi, Qiyue Tan, Ruizhi Zhang, Mei Yue, Rongshuang Ma, Qiang Chen, Shu-hua Zhao, L. Yang

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引用次数: 2

Abstract

INTRODUCTION The effects of ethephon on the reproductive systems of mammalian females are still ambiguous. This study was conducted to evaluate the toxic effects of ethephon on the female reproductive system. MATERIAL AND METHODS Forty female C57 mice were used as experimental subjects and evenly divided into 8 groups, which were fed with mixed ethephon (0, 107.3, 214.5, and 429 mg/kg bw/day) and pure water. After 20 and 40 days of gavage, the mice were weighed and individual organ coefficients of the ovaries were measured. Enzyme-linked immunoassay was used to detect the serum levels of serum sex hormones. The cell cycle distribution and rate of apoptosis of mouse ovarian tissues were examined using flow cytometry. RESULTS Ethephon intoxication significantly decreased serum levels of progesterone (P) and oestradiol (E2) and increased the serum levels of luteinizing hormone (LH). The serum levels of follicle-stimulating hormone (FSH) decreased and then increased over time. In addition, ethephon significantly inhibited the apoptosis rate in the ovary and caused G0/G1 and G2/M arrest. CONCLUSION These results indicate that prolonged exposure to ethephon may have negative effects on the female reproductive system.

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乙烯利对小鼠血清性激素水平、卵巢细胞凋亡及细胞周期的影响。

乙烯利对哺乳动物雌性生殖系统的影响尚不清楚。本研究旨在评估乙烯利对雌性生殖系统的毒性作用。材料与方法以雌性C57小鼠40只为实验对象，随机分为8组，分别饲喂混合乙烯利(0、107.3、214.5、429 mg/kg bw/d)和纯净水。灌胃20、40 d后称重，测定卵巢各脏器系数。采用酶联免疫分析法检测血清性激素水平。采用流式细胞术检测小鼠卵巢组织细胞周期分布及凋亡率。结果沙利中毒大鼠血清黄体酮(P)、雌二醇(E2)水平显著降低，黄体生成素(LH)水平显著升高。随着时间的推移，血清促卵泡激素(FSH)水平先下降后上升。此外，乙烯利显著抑制卵巢细胞凋亡率，导致G0/G1和G2/M阻滞。结论长期接触乙烯利可能对女性生殖系统产生不良影响。

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来源期刊

Endokrynologia Polska ENDOCRINOLOGY & METABOLISM-

CiteScore

2.60

自引率

9.50%

发文量

129

审稿时长

6-12 weeks

期刊介绍： "Endokrynologia Polska" publishes papers in English on all aspects of clinical and experimental endocrinology. The following types of papers may be submitted for publication: original articles, reviews, case reports, postgraduate education, letters to the Editor (Readers’ Forum) and announcements of scientific meetings, conferences and congresses.