Silver nitrate and silver-thiosulphate mitigates callus and leaf abscission during Shisham clonal micro-propagation

Q3 Agricultural and Biological Sciences

Journal of Plant Biotechnology Pub Date : 2021-09-30 DOI:10.5010/jpb.2021.48.3.173

Manoj Raturi, A. Thakur

引用次数: 1

Abstract

Basal callus formation and leaf abscission is a problem in clonal micropropagation. We have described an in vitro clonal propagation protocol of Dalbergia sissoo Roxb (shisham) ‘FRI-14’ in which AgNO 3 played important role not only in mitigating problem of leaf abscission and basal callus, but also improved shoot induction and multiplication. Best induction and shoot multiplication was obtained on MS media with 1.5 mg/l 6-BAP and 10 mg/l AgNO 3 and half-strength MS media with 0.5 mg/l 6-BAP, 2 mg/l AgNO 3 and 50 mg/l Adenine sulphate whereas best ex vitro rooting was obtained with 200 mg/l IBA in pulse treatment.

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硝酸银和硫代硫酸银可减轻水参无性系繁殖过程中愈伤组织和叶片脱落

基部愈伤组织的形成和叶片脱落是无性系繁殖中的一个问题。本文报道了黄檀(Dalbergia sissoo Roxb, shisham) ' fr -14 '的体外克隆繁殖方案，其中AgNO 3在缓解叶片脱落和基部愈伤组织问题的同时，还能促进茎的诱导和增殖。以1.5 mg/l 6-BAP + 10 mg/l agno3的MS培养基和0.5 mg/l 6-BAP + 2 mg/l agno3 + 50 mg/l硫酸腺嘌呤的半强MS培养基诱导和增殖效果最好，以200 mg/l IBA脉冲处理的离体生根效果最好。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Plant Biotechnology Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

1.20

自引率

0.00%

发文量

期刊介绍： Journal of Plant Biotechnology (JPB) is an international open access journal published four issues of a yearly volume on March 31, June 30, September 30, and December 31 by The Korean Society for Plant Biotechnology (KSPBT) founded in 1973. JPB publishes original, peer-reviewed articles dealing with advanced scientific aspects of plant biotechnology, which includes molecular biology, genetics, genomics, proteomics, and metabolomics. JPB does not exclude studies on lower plants including algae and cyanobacteria if studies are carried out within the aspects described above.