Enhancement of Swarming and Inhibition of Prodigiosin in Serratia marcescens by Glyceryl Trinitrate

Q4 Medicine

Journal of Communicable Diseases Pub Date : 2023-05-04 DOI:10.24321/0019.5138.202305

A. Turki

引用次数: 0

Abstract

Introduction:Serratia marcescens is a gram-negative pathogen of many species. Its pathogenicity and survival are linked to its capacity to build biofilms as well as its strong inherent resistance to antimicrobials and cleaning agents. Objectives: To analyse the impact of glyceryl trinitrate (GTN) on the gene expression of QS-related genes (rssB, rsmA,and pigP) of S. marcescens. Methodology: The broth microdilution technique estimated the bactericidal effectiveness of glyceryl trinitrate. The presence of rssB, rsmA,and pigP in S. marcescens isolates was detected using PCR. qRT-PCR was used to assess the effect of GTN on rssB, rsmA,and pigPgene expression. Results: The results demonstrated that GTN has no effect on S. marcescens growth. Furthermore, rssB, rsmA,and pigP were located in all S. marcescens isolates and their gene expression was downregulated due to GTN presence. Conclusion: GTN can act as a promising anti-quorum sensing agent in reference to S. marcescens.

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三硝酸甘油增强粘质沙雷氏菌的群集及抑菌作用

粘质沙雷菌是一种多种革兰氏阴性病原体。它的致病性和存活与它形成生物膜的能力以及它对抗菌剂和清洁剂的强大固有耐药性有关。目的:分析三硝酸甘油(GTN)对粘质葡萄球菌qs相关基因(rssB、rsmA和pigP)基因表达的影响。方法:采用微量肉汤稀释法测定三硝酸甘油的杀菌效果。采用PCR方法检测粘质葡萄球菌分离株中rssB、rsmA和pigP的存在。采用qRT-PCR检测GTN对rssB、rsmA和pigPgene表达的影响。结果:GTN对肉苁蓉生长无明显影响。此外，rssB、rsmA和pigP在所有粘质葡萄球菌分离株中都存在，并且由于GTN的存在，它们的基因表达下调。结论:GTN是一种很有前途的抗聚群感应剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Communicable Diseases Medicine-Infectious Diseases

CiteScore

0.40

自引率

0.00%

发文量

期刊介绍： Journal of Communicable Diseases (E-ISSN: 0019-5138 & P-ISSN: 2394-7047) is published by ADR Publications and is the official publication of Indian Society of Malaria and Other Communicable Diseases. Journal of Communicable Diseases covers scientific researches in the field of communicable diseases. Accept articles with scientific excellence in the form of (1) Original articles in basic and field research (2) Critical reviews, (3) surveys, (4) Case studies, (5) opinions/Correspondence/letters to editor, etc. The first issue of the publication entitled “Bulletin of the National Society of India for Malaria and Other Mosquito-Borne Diseases” the precursor of “Journal of Communicable Disease” (J Commun Dis) was brought out in 1953. The objects and purposes of J Commun Dis are: • to advance knowledge regarding the cause, prevalence, epidemiology, treatment, prevention and control of malaria and other-mosquito-borne diseases and other communicable diseases, • to stimulate scientific and practical interest among individuals and organizations in the prompt and effective application of treatment and control methods, • to integrate scientific and field activities and co-ordinate various scientific investigations, • to disseminate such knowledge both to scientists and to the general public.