{"title":"Effects of high-fat diet on liver function and intestinal bacterial community in rats","authors":"Shi-yong Zhao, Xianyao Lin, Wen Song, Haishao Chen","doi":"10.3760/CMA.J.ISSN.1674-635X.2018.05.007","DOIUrl":null,"url":null,"abstract":"Objective \nTo investigate the influence of high-fat diet on liver function and intestinal bacterial community through building rat models. \n \n \nMethods \n20 rats of 21 days old were divided into two groups randomly as normal diet group fed with standard chow diet and high-fat group fed with high-fat diet. After 6 weeks, feces of rats in both groups were obtained for 16S rRNA high-through sequencing of the intestinal bacterial community. \n \n \nResults \nAfter 6 weeks high-fat diet, total protein (TP)(55.79±3.75, P=0.002), globin (GLB)( 34.9±2.53, P<0.001), albumin (ALB)/GLB (.60±0.02, P<0.001), alkaline phosphatase (ALP)(373.80±63.05, P<0.001), total cholesterol (TC)(1.94±0.23, P<0.001), low density lipoprotein (LDL)(0.76±0.93, P<0.001), LDL/high density lipoprotein (HDL)(1.43±0.22, P<0.001), and triglyceride (TG)(1.48±0.50, P=0.015) increased compared with the normal diet group. Additionally, intestinal bacterial diversity and evenness decreased significantly. The dominant bacteria were Bacteroidetes, Firmicutes, and Proteobacteria, with averaged relative abundances as 56.36%, 35.31%, and 6.61%, respectively. The relative abundances of Bacteroidetes deceased (P=0.007), those of Firmicutes increased (P=0.020), and those of Proteobacteria were kept stable (P=0.928) after a 6-week high-fat diet. Furthermore, the intestinal bacterial community structure changed distinctly between the two groups by 16s rRNA high-through sequencing. \n \n \nConclusion \nHigh-fat diet can lead to change of intestinal bacterial community structure and further result in liver function damnification as well as obesity. \n \n \nKey words: \nHigh-through sequencing; 16S rRNA; Rat; Intestinal bacterial community; Liver function","PeriodicalId":9877,"journal":{"name":"中华临床营养杂志","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2018-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华临床营养杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.1674-635X.2018.05.007","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Nursing","Score":null,"Total":0}
引用次数: 0
Abstract
Objective
To investigate the influence of high-fat diet on liver function and intestinal bacterial community through building rat models.
Methods
20 rats of 21 days old were divided into two groups randomly as normal diet group fed with standard chow diet and high-fat group fed with high-fat diet. After 6 weeks, feces of rats in both groups were obtained for 16S rRNA high-through sequencing of the intestinal bacterial community.
Results
After 6 weeks high-fat diet, total protein (TP)(55.79±3.75, P=0.002), globin (GLB)( 34.9±2.53, P<0.001), albumin (ALB)/GLB (.60±0.02, P<0.001), alkaline phosphatase (ALP)(373.80±63.05, P<0.001), total cholesterol (TC)(1.94±0.23, P<0.001), low density lipoprotein (LDL)(0.76±0.93, P<0.001), LDL/high density lipoprotein (HDL)(1.43±0.22, P<0.001), and triglyceride (TG)(1.48±0.50, P=0.015) increased compared with the normal diet group. Additionally, intestinal bacterial diversity and evenness decreased significantly. The dominant bacteria were Bacteroidetes, Firmicutes, and Proteobacteria, with averaged relative abundances as 56.36%, 35.31%, and 6.61%, respectively. The relative abundances of Bacteroidetes deceased (P=0.007), those of Firmicutes increased (P=0.020), and those of Proteobacteria were kept stable (P=0.928) after a 6-week high-fat diet. Furthermore, the intestinal bacterial community structure changed distinctly between the two groups by 16s rRNA high-through sequencing.
Conclusion
High-fat diet can lead to change of intestinal bacterial community structure and further result in liver function damnification as well as obesity.
Key words:
High-through sequencing; 16S rRNA; Rat; Intestinal bacterial community; Liver function