Phylogenetically distant clade of Nostoc-like taxa with the description of Minunostoc gen. nov. and Minunostoc cylindricum sp. nov

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2019-04-01 DOI:10.5507/FOT.2018.013

Fangfang Cai, Xiaochuang Li, Ruozhen Geng, Xin Peng, Renhui Li

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引用次数: 30

Abstract

Two cyanobacterial strains morphologically identified to the genus Nostoc were isolated from a wet rocky wall in a mid-subtropical region in China, and they were taxonomically and phylogenetically characterized based on the polyphasic approach combining morphological and genetic characteristics. 16S rRNA gene sequence analysis showed that the two strains containing six clones were all >99.6% similar to each other, but had < 94.3% similarities to the existing cyanobacterial genera. The phylogenies based on 16S rRNA and rpoC1 gene sequences indicated that their sequences grouped into a unique and robust cluster with high bootstrap values. This unique cluster was separated from the clade of the 'Nostoc sensu stricto' and the respective clades formed by the morphologically similar genera Mojavia, Desmonostoc, Aliinostoc, Komarekiella and Halotia. The 16S-23S rRNA ITS secondary structure of the both strains exhibited the unique pattern of D1-D1´, Box-B and V3 helix, distinguishing it from the other heterocytous genera. Such a clear cluster leads to the establishment of Minunostoc gen. nov., with the type species as Minunostoc cylindricum sp. nov.

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具有Minunostoc gen. 11和Minunostoc icum sp. nov描述的nostoc -样分类群的系统发育远分支

从中国中亚热带潮湿的岩壁上分离到两株形态鉴定为发菜属的蓝藻菌株，并采用形态学和遗传学相结合的多相方法对其进行了分类和系统发育特征鉴定。16S rRNA基因序列分析表明，包含6个克隆的两个菌株彼此的相似性均>99.6%，但与现有的蓝藻属的相似性<94.3%。基于16S rRNA和rpoC1基因序列的系统发育表明，它们的序列组成了一个独特而稳健的簇，具有较高的自举值。这个独特的集群是从“严格意义上的Nostoc”的分支和形态相似的Mojavia属、Desmonostoc属、Aliinostoc属、Komarekiella属和Halotia属形成的各自分支中分离出来的。这两个菌株的16S-23S rRNA ITS二级结构都表现出独特的D1-D1´、Box-B和V3螺旋模式，将其与其他异源细胞属区分开来。这样一个清晰的聚类导致了Minunostoc gen.nov.的建立，模式种为Minunostoch cylindrium sp.nov。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.