Evaluation of free amino acids in Cirsium vulgare (Bull thistle) plant materials using gas chromatography–mass spectrometry

IF 0.5 4区化学 Q4 CHEMISTRY, MULTIDISCIPLINARY

Chemija Pub Date : 2022-08-07 DOI:10.6001/chemija.v33i3.4749

U. Griškevičienė, M. Marksa, A. Ževžikovienė, A. Ževžikovas, L. Ivanauskas

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Abstract

Amino acids are one of the main particles in a living organism. They perform many different functions, such as structural, receptor, energetic and enzymatic functions [1]. The aim of the investigation was to identify and evaluate the qualitative composition of free amino acids in samples of Cirsium vulgare material using gas chromatography–mass spectrometry (GC-MS). Our objectives were to apply the gas chromatography– mass spectrometry (GC-MS) method for free amino acids from Cirsium vulgare raw materials grown in Lithuania and determine the qualitative composition of free amino acids from extracts of Cirsium vulgare raw materials: roots, leaves, flowers and seeds. MTBSTFA (N-methyl-N-(tertbutyldimethylsilyl) trifluoroacetamide) was selected for derivatisation in free amino acid analysis. Applying gas chromatography–mass spectrometry with compound derivatisation and based on the mass spectral database free amino acids were identified in bull thistle raw material: L-alanine, glycine, L-valine, L-leucine, L-isoleucine, L-proline, L-serine, L-threonine, L-phenylalanine, L-lysine, L-tyrosine, L-aspartic acid and L-glutamic acid. Studies have shown that the qualitative composition of free amino acids depends on the plant vegetation phase and plant parts.

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气相色谱-质谱法评价牛蓟植物原料中游离氨基酸

氨基酸是生物体内的主要颗粒之一。它们具有许多不同的功能，如结构、受体、能量和酶功能[1]。本研究的目的是使用气相色谱-质谱联用技术（GC-MS）鉴定和评价Cirsium vulgare材料样品中游离氨基酸的定性组成。我们的目标是应用气相色谱-质谱法（GC-MS）测定立陶宛种植的小白菜原料中的游离氨基酸，并测定小白菜原料提取物（根、叶、花和种子）中游离氨基酸的定性组成。在游离氨基酸分析中选择MTBSTFA（N-甲基-N-（叔丁基二甲基甲硅烷基）三氟乙酰胺）进行衍生化。应用化合物衍生的气相色谱-质谱法，并基于质谱数据库，在牛蓟原料中鉴定出游离氨基酸：L-丙氨酸、甘氨酸、L-缬氨酸、L-亮氨酸、L-异亮氨酸、L-脯氨酸、L-丝氨酸、L-苏氨酸、L-苯丙氨酸、L-赖氨酸、L-酪氨酸、L-天冬氨酸和L-谷氨酸。研究表明，游离氨基酸的定性组成取决于植物的植被阶段和植物部位。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemija 化学-化学综合

CiteScore

1.30

自引率

16.70%

发文量

审稿时长

>12 weeks

期刊介绍： Chemija publishes original research articles and reviews from all branches of modern chemistry, including physical, inorganic, analytical, organic, polymer chemistry, electrochemistry, and multidisciplinary approaches.