Seed pretreatment for control of powdery mildew infection on purple ipe micropropagation

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-04-01 DOI:10.1590/2447-536x.v28i2.2360

Talita Cristina Mamedes, A. A. Chaibub, K. C. I. Sousa, M. T. Faria, Letícia de Almeida Gonçalves, M. C. Filippi, L. G. Araújo, S. T. Sibov

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Abstract

Abstract Purple ipe (Handroanthus impetiginosus) is an important tree species in Cerrado biome conservation and very popular at the landscaping and urban afforestation. However, its micropropagation is affected by pathogens, such as Oidium sp. The aim this study was evaluate the efficiency of seed treatments in the control of powdery mildew of purple ipe obtained by micropropagation. The symptoms were observed during in vitro germination, a Koch’s postulates were performed for confirm the pathogenicity, colonization of the pathogen on the leaves was analyzed in optical and scanning microscopes and a scale to evaluate severity was proposed. Two experiments were realized to powdery mildew control using a completely randomized design, with 30 replicates. First experiment: Seeds were treated with ethanol (Et), chlorothalonil + thiophanate-methyl (C+TM), and sodium hypochlorite (NaOCl); second experiment: Seeds were treated with Et, NaOCl, C+TM, and neem oil. Disease severity and area under the disease progress curve (AUDPC) were assessed in both experiments. Disease symptoms and typical pathogen structures were observed, and the pathogenicity was confirmed. The disease severity was reduced by 30.78% in 1.5% neem oil for 10 min when compared with C+TM for 15 min. We conclude that neem oil can be a strategy sustainable for the control of powdery mildew in purple ipe in tissue culture.

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种子预处理防治紫管微繁殖中的白粉菌感染

紫ipe (Handroanthus impetiginosus)是塞拉多地区重要的生物群落保护树种，在园林绿化和城市绿化中非常受欢迎。但其微繁易受病原菌的影响，如Oidium sp.。本研究的目的是评价种子处理对紫ipe微繁获得的白粉病的防治效果。在离体萌发过程中观察病原菌的症状，采用科赫定律确定病原菌的致病性，在光学显微镜和扫描显微镜下分析病原菌在叶片上的定殖情况，并提出了病原菌严重程度的评分标准。采用完全随机设计进行2个白粉病防治试验，共30个重复。第一个实验:用乙醇(Et)、百菌清+甲基硫代盐(C+TM)和次氯酸钠(NaOCl)处理种子;第二个实验:用Et、NaOCl、C+TM和楝油处理种子。在两个实验中都评估了疾病严重程度和疾病进展曲线下面积(AUDPC)。观察疾病症状和典型病原菌结构，确定致病性。与C+TM处理15 min相比，1.5%印楝油处理10 min可使紫豆白粉病的严重程度降低30.78%。由此可见，在紫豆组织培养中，印楝油是一种可持续的防治策略。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.