Decreased level of the anti-inflammatory adipokines, secreted frizzled-related protein 5 and adiponectin, in high cholesterol diet-induced atherosclerotic rats

A. Gharibi, P. Yaghmaei, G. Basati, K. Soleimannejad, N. Abbasi
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Abstract

Introduction: The involvement of secreted frizzled-related protein5 (SFRP5) and adiponectin, two important adipokines produced by adipocytes, in cardiovascular diseases demand further assessment. Therefore, in this study the relation of the adipokines and atherosclerosis was evaluated in Rat. Materials and methods: For the study, thirty male Wistar rats were divided into 2 groups (each group contain 15 rats): Control group, received a normal diet and the high cholesterol diet (HCD) group which received an additional 2% cholesterol and 0.5% cholic acid for 15 weeks. At the end of treatment, HCD-induced atheroma plaques were observed by hematoxylin and eosin staining of aortic tissue sections. Furthermore, serum levels of SFRP5 and adiponectin in the two groups of rats were measured by immunoassay and their relationships with the development of atherosclerotic plaques in the experimental group were analyzed. Results: The serum level of SFRP5 and adiponectin was significantly decreased in HCD rats compared with the control group (P<0.05).  There was also an inverse relation between the serum level of the two adipokines and atherosclerotic plaque formation (P<0.05).  Conclusion: Serum levels of SFRP5 and adiponectin are decreased in rats fed with high cholesterol diet, highlighting the involvement of the two adipokines in atherosclerosis.
高胆固醇饮食诱导的动脉粥样硬化大鼠抗炎脂肪因子水平降低,分泌卷曲相关蛋白5和脂联素
分泌性卷曲相关蛋白5 (SFRP5)和脂联素是脂肪细胞产生的两种重要的脂肪因子,它们在心血管疾病中的作用有待进一步研究。因此,本研究探讨了脂肪因子与大鼠动脉粥样硬化的关系。材料与方法:将30只雄性Wistar大鼠分为2组(每组15只):对照组给予正常饮食,高胆固醇饮食(HCD)组给予2%胆固醇和0.5%胆酸,持续15周。治疗结束时,通过苏木精和伊红染色主动脉组织切片观察hcd诱导的动脉粥样硬化斑块。免疫法测定两组大鼠血清SFRP5和脂联素水平,分析实验组SFRP5和脂联素与动脉粥样硬化斑块发生的关系。结果:与对照组相比,HCD大鼠血清SFRP5、脂联素水平显著降低(P<0.05)。血清两种脂肪因子水平与动脉粥样硬化斑块形成呈负相关(P<0.05)。结论:高胆固醇饮食大鼠血清中SFRP5和脂联素水平降低,表明这两种脂肪因子在动脉粥样硬化中的作用。
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