Analysis of polymorphic loci of candidate genes in patients with occupational diseases

Abstract

Introduction. Development and progress features of vibration syndrome (VS) and noise-induced hearing loss (NIHL) are determined by genetic factors to a certain extent. The latter define the relevance of the search for individual approaches to  prevention and medical support of patients with these occupational diseases. The aim of the study was to investigate the genotypes distribution of polymorphic loci of candidate genes in patients with occupational diseases caused by vibration and noise impact. Materials and methods. Polymorphic variants of the HSPA1B (rs1061581), IL17A (rs2275913), IL10 (rs1800872), and TNF-α (rs1800629) genes of three hundred ninety eight males were studied using PCR-RFLP and real-time PCR methods, including 268  patients with VS divided into two groups: VShtv (n = 117) and VScomb (n = 151), in which VS was caused by exposure to hand-transmitted vibration (HTV) or combined exposure to HTV and whole-body vibration (COMB), respectively. The study also involved 96 patients with occupational NIHL diagnosis and 34 individuals of the comparison group without occupational contact with physical factors. Results. The increase in GG-HSPA1B (1267A/G) genotype frequency  (p=0.058) was found in VScomb patients compared with those from the VShtv group. There was a significant difference in carriers of the C/C genotype of the IL10 (rs1800872) polymorphic locus in the group of NIHL patients relative to the comparison group (p=0.036). The results of regression analysis indicate the carriage of the A allele to reduce the risk of NIHL development. Limitations. The limitations include the number of examined cases in all groups, especially in comparison group and the lack of groups of workers with no occupational exposure to a physical factor.   Conclusion. The obtained results expand the understanding of the genetic features able to determine sensitivity to the physical factors exposure that define the development of occupational pathology and the issues to be resolved at the next stage of research.