Effect of enteral nutrition and probiotics on inflammatory factors and immune function in aplastic anemia rats

Q4 Nursing

中华临床营养杂志 Pub Date : 2019-06-30 DOI:10.3760/CMA.J.ISSN.1674-635X.2019.03.010

Yi Wang, P. Shan

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Abstract

Objective To investigate the effect of enteral nutrition and probiotics on inflammatory factors and immune function in aplastic anemia (AA) rats. Methods AA rats model were established by intraperitoneal injection of fluorouracil and lavage Maryland, and the successful AA rats model were randomly divided into model group, enteral nutrition group, probiotics group and combination group, 10 rats in each group. Another 10 Wistar rats were selected as blank group. Rats in the blank group and model group were given saline lavage (same volume 0.9% NaCl), rats in the enteral nutrition group were given Peptisorb nutrient solution [10 g/(kg·d)] by lavage, rats in the probiotics group were given live Clostridium butyricum [2 g/(kg·d)] by lavage, and rats in the combination group were given Peptisorb nutrient solution [10 g/(kg·d)] and live Clostridium butyricum [2 g/(kg·d)] by lavage for 4 weeks. The pathological structure of the bone marrow were observed by HE staining, the contents of white blood cells (WBC), red blood cell (RBC), platelet (PLT) and hemoglobin (Hb) in blood cell were detected by analyzer, the levels of serum tumor necrosis factor alpha (TNF-α), interleukin-2 (IL-2) and interleukin-11 (IL-11) were detected by ELISA method, and the contents of CD4+, CD8+ and NKT cells were detected by flow cytometry instrument. Results The contents of WBC, RBC, PLT and Hb in the model group were significantly lower than that in the blank group, the contents of WBC, RBC, PLT and Hb in enteral nutrition group, probiotics group and combination group were significantly higher than that in the model group, and the combination group was better than the enteral nutrition group and probiotics group (P 0.05). The level of TNF-α and IL-2 were significantly higher and the IL-11 level was significantly lower in the model group than in the blank group, the level of TNF-α and IL-2 were significantly lower and IL-11 level were significantly higher in the enteral nutrition group, probiotics group and combination group than in the model group, and the combination group was better than the enteral nutrition group and probiotics group (P 0.05). The contents of CD4+ and NKT cells were significantly lower and the CD8+ cells was significantly higher in the model group than in the blank group, the level of CD4+ and NKT cells were significantly higher and CD8+ cells were significantly lower in enteral nutrition group, probiotics group and combination group than in the model group, and the combination group was better than the enteral nutrition group and probiotics group (P 0.05). Conclusion Compared with normal consumption or oral glucose, the enteral nutrition combined with probiotics can reduce inflammatory response and improve bone marrow hematopoietic function and immune function in AA rats. Key words: Aplastic anemia; Enteral nutrition; Probiotics; Inflammation factors; Immune function

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肠内营养和益生菌对再生障碍性贫血大鼠炎症因子和免疫功能的影响

目的探讨肠内营养和益生菌对再生障碍性贫血(AA)大鼠炎症因子及免疫功能的影响。方法采用氟尿嘧啶腹腔注射并灌洗Maryland法建立AA大鼠模型，将成功建立的AA大鼠模型随机分为模型组、肠内营养组、益生菌组和联合组，每组10只。另取10只Wistar大鼠作为空白组。空白组和模型组大鼠灌胃生理盐水(同体积0.9% NaCl)，肠内营养组大鼠灌胃胃补菌营养液[10 g/(kg·d)]，益生菌组大鼠灌胃活丁酸梭菌[2 g/(kg·d)]，联合组大鼠灌胃胃补菌营养液[10 g/(kg·d)]和活丁酸梭菌[2 g/(kg·d)]，持续4周。HE染色观察骨髓病理结构，分析仪检测白细胞(WBC)、红细胞(RBC)、血小板(PLT)和血红蛋白(Hb)含量，ELISA法检测血清肿瘤坏死因子α (TNF-α)、白细胞介素-2 (IL-2)和白细胞介素-11 (IL-11)水平，流式细胞仪检测CD4+、CD8+和NKT细胞含量。结果模型组小鼠WBC、RBC、PLT和Hb含量均显著低于空白组，肠内营养组、益生菌组和联合组小鼠WBC、RBC、PLT和Hb含量均显著高于模型组，且联合组优于肠内营养组和益生菌组(P < 0.05)。模型组大鼠血清TNF-α和IL-2水平显著高于空白组，IL-11水平显著低于空白组，肠内营养组、益生菌组和联合组大鼠血清TNF-α和IL-2水平显著低于模型组，IL-11水平显著高于模型组，且联合组优于肠内营养组和益生菌组(P 0.05)。模型组小鼠CD4+和NKT细胞含量显著低于空白组，CD8+细胞含量显著高于空白组，肠内营养组、益生菌组和联合组小鼠CD4+和NKT细胞水平显著高于模型组，CD8+细胞水平显著低于模型组，且联合组优于肠内营养组和益生菌组(P 0.05)。结论与正常摄入或口服葡萄糖相比，肠内营养联合益生菌可降低AA大鼠的炎症反应，改善骨髓造血功能和免疫功能。关键词:再生障碍性贫血;肠内营养;益生菌;炎症因素;免疫功能

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来源期刊

中华临床营养杂志 Nursing-Nutrition and Dietetics

CiteScore

0.20

自引率

0.00%

发文量

2282

期刊介绍： The Chinese Journal of Clinical Nutrition was founded in 1993. It is the first professional academic journal (bimonthly) in my country co-sponsored by the Chinese Medical Association and the Chinese Academy of Medical Sciences to disseminate information on clinical nutrition support, nutrient metabolism, the impact of nutrition support on outcomes and "cost-effectiveness", as well as translational medicine and nutrition research. It is also a professional journal of the Chinese Medical Association's Parenteral and Enteral Nutrition Branch. The purpose of the Chinese Journal of Clinical Nutrition is to promote the rapid dissemination of knowledge on nutrient metabolism and the rational application of parenteral and enteral nutrition, focusing on the combination of multidisciplinary and multi-regional field investigations and clinical research. It mainly reports on nutritional risk screening related to the indications of parenteral and enteral nutrition support, "cost-effectiveness" research on nutritional drugs, consensus on clinical nutrition, guidelines, expert reviews, randomized controlled studies, cohort studies, glycoprotein and other nutrient metabolism research, systematic evaluation of clinical research, evidence-based case reports, special reviews, case reports and clinical experience exchanges, etc., and has a special column on new technologies related to the field of clinical nutrition and their clinical applications.