Establishment of in vitro regeneration system for genome editing in potato cv. Lady Rosetta

IF 0.7 4区农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY

Pakistan Journal of Agricultural Sciences Pub Date : 2021-11-01 DOI:10.21162/pakjas/21.651

Hafiza Arooj Razaq

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引用次数: 1

Abstract

The genome engineering methods have gained much appreciation for hitting plant disease resistance panorama since the last decade. The in vitro regeneration system is the foundation part of any research related to genetic engineering in plants. Hence, we established a tissue culture regime for efficient in vitro regeneration response of potato cultivar Lady Rosetta tagged with low to no resistance to late blight on foliage and medium to low resistance to late blight on tubers in potato. In this study, five callus induction media and seven regeneration media were investigated to get the best in vitro regeneration response in Lady Rosetta. Based on a statistical analysis of recorded data, the selected combination was CIM2-28 days old calli-RM7. The genetic fidelity of in vitro regenerated plants on a selected combination was assessed using the SSR markers. The wild-type and in vitro regenerated plants showed the same DNA profile. Moreover, a minimal inhibitory dose of basta was optimized for selecting genetically transformed plants in potato, and a 9mg /L basta dose was selected.

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马铃薯罗塞塔夫人基因组编辑离体再生体系的建立

近十年来，基因组工程方法因其对植物抗病性的全面研究而备受赞誉。体外再生系统是任何与植物基因工程相关的研究的基础部分。因此，我们建立了一种组织培养制度，用于对马铃薯品种Lady Rosetta进行有效的体外再生反应，该品种对叶片晚疫病具有低至无抗性，对块茎晚疫病具有中至低抗性。在本研究中，对五种愈伤组织诱导培养基和七种再生培养基进行了研究，以获得罗塞塔夫人最佳的体外再生反应。基于对记录数据的统计分析，选择的组合是CIM2-28日龄的愈伤组织-RM7。使用SSR标记评估体外再生植物对所选组合的遗传保真度。野生型和体外再生植物显示出相同的DNA图谱。此外，还优化了巴斯塔的最小抑制剂量来选择转基因植株，并选择了9mg/L的巴斯塔剂量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pakistan Journal of Agricultural Sciences AGRICULTURE, MULTIDISCIPLINARY-

CiteScore

1.80

自引率

25.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Pakistan Journal of Agricultural Sciences is published in English four times a year. The journal publishes original articles on all aspects of agriculture and allied fields.