Combined effect of corn in the barrier crop and plant extracts against Cowpea mild mottle virus infecting soybean [Glycine max (L.) Merr.] in the field

IF 0.6 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY
W. R. Andayanie, M. Lukito, N. Ermawati, Wuye Ria, Andayanie
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Abstract

Cowpea mild mottle virus (CpMMV) is one of the problems that can decrease soybean production. The research was conducted on the combined effects of corn in the barrier crop with plant extracts against CpMMV infecting soybean in the field. The field data was conducted using a Completely Randomized Design. The mean of disease incidence and disease severity is measured from total plants in each replicate plot on each treatment. Planting one or two of corn lines were grown at the edge four weeks before planting soybeans. Cashew nut shell (CNS), pagoda leaf, and rhizome of ginger extracts were applied using the sprayer and applied at 24 h before virus acquisition and transmission by whiteflies. The result showed that the virus incubation period ranged from 9−38 days after transmission longer than the untreated control. Planting two  corn lines at the edge with CNS extract as  bioactivator on soybean was the most extended incubation period of the virus and the lowest absorbance value DAS-ELISA of 0.20. There was a 73.11 % increase in the relative inhibition level of the virus. Planting  corn at the edge with CNS extract proved to be more effective than soybean monoculture with CNS extract. However, soybean monoculture with CNS extract provides a better relative inhibition level of the virus (64.32 %) than planting two rows of corn on the  edge combined with ginger of rhizome extract and planting two rows of corn on the edge with pagoda leaf extract as bioactivator on a soybean plant.
屏障作物玉米与植物提取物联合防治大豆轻度斑驳病毒的研究稳定。在野外
豇豆轻度斑点病毒(CpMMV)是大豆产量下降的主要原因之一。研究了玉米与植物提取物对大豆感染CpMMV的联合效应。现场数据采用完全随机设计进行。疾病发生率和疾病严重程度的平均值是从每次处理的每个复制区的总植物中测量的。在种植大豆前四周,在边缘种植一到两个玉米系。使用喷雾器施用腰果壳(CNS)、宝塔叶和生姜根茎提取物,并在病毒被白粉虫获取和传播前24小时施用。结果表明,与未经治疗的对照组相比,病毒传播后的潜伏期从9−38天不等。在大豆上以中枢神经系统提取物为生物活性剂在边缘种植两个玉米系是病毒潜伏期最长的,DAS-ELISA的吸光度值最低,为0.20。病毒的相对抑制水平增加了73.11%。用CNS提取物在边缘种植玉米被证明比用CNS提取物单一种植大豆更有效。然而,与在大豆植株上在边缘种植两排玉米结合根茎提取物和在边缘种植二排玉米结合宝塔叶提取物作为生物活性剂相比,使用中枢神经系统提取物的大豆单一栽培提供了更好的病毒相对抑制水平(64.32%)。
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来源期刊
Bioscience Journal
Bioscience Journal Agricultural and Biological Sciences-General Agricultural and Biological Sciences
CiteScore
1.00
自引率
0.00%
发文量
90
审稿时长
48 weeks
期刊介绍: The Bioscience Journal is an interdisciplinary electronic journal that publishes scientific articles in the areas of Agricultural Sciences, Biological Sciences and Health Sciences. Its mission is to disseminate new knowledge while contributing to the development of science in the country and in the world. The journal is published in a continuous flow, in English. The opinions and concepts expressed in the published articles are the sole responsibility of their authors.
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