{"title":"Isolation and characterization of Rep PepYLCV encoding gene from West Sumatra","authors":"Bastian Nova, E. Syafriani, A. Asben, J. Jamsari","doi":"10.21475/poj.11.01.18.pne1055","DOIUrl":null,"url":null,"abstract":"Replicase (Rep) protein of Geminivirus is known as one of the important components not only for its successful replication in their host but also known to interact with various host plant proteins. However, it is still unclear if those interactions are associated with symptoms level. This research aims to explore the possibility of Rep as pathogenic determinant by in silico approach. Here we report the comparison of three Rep sequences isolated from Pesisir Selatan and Tanah Datar districts in West Sumatra Indonesia. The PCR-based cloning approach was used in this study to isolate the gene sequences from all isolates. Pathogenic determinant was predicted from phenotype and genotype analysis. Phenotype data showed symptoms appearance after 8 dpi for PSSWS14 and 20 dpi for PSSWS3. Furthermore, genotype showed that the nonconserved region in N-terminal of Rep makes different in its putative binding site. It is prospective to be related to the symptoms appearance rates. We predict the differences in N-terminal of Rep affecting the symptoms appearance rates of Geminivirus infection.","PeriodicalId":54602,"journal":{"name":"Plant Omics","volume":"23 15","pages":"37-41"},"PeriodicalIF":0.0000,"publicationDate":"2018-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Omics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21475/poj.11.01.18.pne1055","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 1
Abstract
Replicase (Rep) protein of Geminivirus is known as one of the important components not only for its successful replication in their host but also known to interact with various host plant proteins. However, it is still unclear if those interactions are associated with symptoms level. This research aims to explore the possibility of Rep as pathogenic determinant by in silico approach. Here we report the comparison of three Rep sequences isolated from Pesisir Selatan and Tanah Datar districts in West Sumatra Indonesia. The PCR-based cloning approach was used in this study to isolate the gene sequences from all isolates. Pathogenic determinant was predicted from phenotype and genotype analysis. Phenotype data showed symptoms appearance after 8 dpi for PSSWS14 and 20 dpi for PSSWS3. Furthermore, genotype showed that the nonconserved region in N-terminal of Rep makes different in its putative binding site. It is prospective to be related to the symptoms appearance rates. We predict the differences in N-terminal of Rep affecting the symptoms appearance rates of Geminivirus infection.
期刊介绍:
Plant OMICS is an international, peer-reviewed publication that gathers and disseminates fundamental and applied knowledge in almost all area of molecular plant and animal biology, particularly OMICS-es including:
Coverage extends to the most corners of plant and animal biology, including molecular biology, genetics, functional and non-functional molecular breeding and physiology, developmental biology, and new technologies such as vaccines. This journal also covers the combination of many areas of molecular plant and animal biology. Plant Omics is also exteremely interested in molecular aspects of stress biology in plants and animals, including molecular physiology.