Analysis of Genetic Diversity in Wild and Domesticated Stocks of the Tropical Abalone Haliotis asinina by Microsatellite Polymorphism

Q4 Agricultural and Biological Sciences

Genetics of Aquatic Organisms Pub Date : 2022-06-02 DOI:10.4194/ga501

P. Praipue, S. Klinbunga, Sirikan Prasertlux, Sirithorn Janpoom, Puttawan Rongmung, Ornchuda Ratdee, Wanwipa Ittarat, Phimsucha Boonphimpapha, P. Jarayabhand, B. Khamnamtong

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Abstract

The basic information on genetic diversity of wild and hatchery-propagated stocks of tropical abalone, Haliotis asinina is important for the construction of a breeding scheme leading to the sustainable culturing activity of this species. In this study, 2,876 expressed sequence tags (ESTs) were in silico analyzed and 178 EST sequences contained microsatellite motifs. Four loci (DW455, DW503, PHe177, and PT102) of type I and two loci (Haμ9 and Haμ10) of type II microsatellites were applied for genetic diversity studies. The mean number of alleles per locus and observed heterogeneity in wild populations were 4.167 and 0.483, and 4.833 and 0.528 for CAME (east coast) and TRGW (west coast) while those of hatchery-propagated samples were 3.000 and 0.708, 4.500 and 0.479, 5.167 and 0.524, and 6.333 and 0.527 for PHIH (2nd generation, G2; Philippines), SAMH (G1), SMaRT-TRGH (G8) and SMaRT-SICH (G8), respectively. The numbers of alleles, observed heterozygosity and effective population sizes suggested no severe reduction of genetic diversity in our breeding program of H. asinina. However, reduced Ne was observed in the cultured stocks from the Philippines (PHIH). FST-statistics and the exact test between pairs of samples revealed significant genetic differences between all pairwise comparisons of samples in this study.

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应用微卫星多态性分析热带鲍野生和驯化种群的遗传多样性

关于野生和孵化繁殖的热带鲍鱼种群遗传多样性的基本信息对于构建该物种可持续培养活动的育种方案至关重要。在本研究中，对2876个表达序列标签（EST）进行了计算机分析，178个EST序列包含微卫星基序。应用四个I型微卫星基因座（DW455、DW503、PHe177和PT102）和两个II型微卫星的基因座（Haμ9和Haμ10）进行遗传多样性研究。CAME（东海岸）和TRGW（西海岸）的每个基因座的平均等位基因数和野生种群中观察到的异质性分别为4.167和0.483，4.833和0.528，而PHIH（第二代，G2；菲律宾）、SAMH（G1）、SMaRT TRGH（G8）和SMaRT SICH（G8）的孵化繁殖样本的等位基因平均数分别为3.000和0.708，4.500和0.479，5.167和0.524，6.333和0.527。等位基因的数量、观察到的杂合性和有效种群规模表明，在我们的H.asinina育种计划中，遗传多样性没有严重降低。然而，在来自菲律宾（PHIH）的培养物中观察到Ne减少。FST统计数据和成对样本之间的精确检验揭示了本研究中所有成对样本比较之间的显著遗传差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genetics of Aquatic Organisms Agricultural and Biological Sciences-Aquatic Science

CiteScore

0.90

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0.00%

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