CRISPR-Cas-Based Engineering of Probiotics.

Q2 Agricultural and Biological Sciences
生物设计研究(英文) Pub Date : 2023-09-29 eCollection Date: 2023-01-01 DOI:10.34133/bdr.0017
Ling Liu, Shimaa Elsayed Helal, Nan Peng
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引用次数: 1

Abstract

Probiotics are the treasure of the microbiology fields. They have been widely used in the food industry, clinical treatment, and other fields. The equivocal health-promoting effects and the unknown action mechanism were the largest obstacles for further probiotic's developed applications. In recent years, various genome editing techniques have been developed and applied to explore the mechanisms and functional modifications of probiotics. As important genome editing tools, CRISPR-Cas systems that have opened new improvements in genome editing dedicated to probiotics. The high efficiency, flexibility, and specificity are the advantages of using CRISPR-Cas systems. Here, we summarize the classification and distribution of CRISPR-Cas systems in probiotics, as well as the editing tools developed on the basis of them. Then, we discuss the genome editing of probiotics based on CRISPR-Cas systems and the applications of the engineered probiotics through CRISPR-Cas systems. Finally, we proposed a design route for CRISPR systems that related to the genetically engineered probiotics.

Abstract Image

Abstract Image

基于CRISPR-Cas的益生菌工程。
益生菌是微生物学领域的瑰宝。它们已被广泛应用于食品工业、临床治疗等领域。模棱两可的健康促进作用和未知的作用机制是益生菌进一步发展应用的最大障碍。近年来,人们开发并应用了各种基因组编辑技术来探索益生菌的作用机制和功能修饰。作为重要的基因组编辑工具,CRISPR-Cas系统为益生菌基因组编辑开辟了新的改进。CRISPR-Cas系统具有高效、灵活、特异等优点。在这里,我们总结了CRISPR-Cas系统在益生菌中的分类和分布,以及在此基础上开发的编辑工具。然后,我们讨论了基于CRISPR-Cas系统的益生菌基因组编辑以及通过CRISPR-Cas系统工程化益生菌的应用。最后,我们提出了一条与基因工程益生菌相关的CRISPR系统的设计路线。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
3.90
自引率
0.00%
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0
审稿时长
12 weeks
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