Impact of Nanographene Oxide on Cisplatin Induced Acute Kidney Injury Managed by Stem Cells Therapy.

IF 0.8 4区医学 Q4 UROLOGY & NEPHROLOGY

Iranian journal of kidney diseases Pub Date : 2023-09-01

Parvin Karimzadeh, Tahereh Foroutan, Mohsen Nafar, Sahar Kalavati

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引用次数: 0

Abstract

Introduction: Graphene-based nanomaterials have shown some degrees of stem cell protection against cell death. Due to their distinctive function, the kidneys are exposed to many toxic substances. On the other hand, minor and trivial effects of stem cells have been reported for the treatment of acute kidney injury (AKI). Here, we explain the use of Graphene oxide (GO) for improving the efficacy of mesenchymal stem cells (MSCs) in the treatment of Cisplatin-induced AKI.

Methods: In this study, GO particles were synthesized in our lab. Cisplatin-induced AKI was modeled on rats. Thirty adults male Wistar Albino rats were divided into five groups: control group (did not receive any treatment), Cisplatin group (received 5 mg/ kg cisplatin intraperitoneally), sham group (received 500 µL saline intraperitoneally 5th days after Cisplatin injection), [Cisplatin + MSCs] group (received 5×106 /kg MSCs after Cisplatin injection), and [Cisplatin+ MSCs + GO] group (received 1.5 mg/kg GO + MSCs after Cisplatin injection. Biochemical analysis of serum creatinine (Cr) and blood urea nitrogen (BUN) levels, as well as histological study of the kidneys in diverse groups were compared. The oneway analysis of variance (ANOVA) and Dunnett's test were used for comparisons between the study groups.

Results: GO improved the effects of MSCs transplantation on serum Cr and BUN in AKI rat models. It also reduced cell death, hyaline casts, and cell debris in the animal models compared to the MSCs group.

Conclusion: It could be concluded that GO can enhance the efficacy of MSCs transplantation in the treatment of damaged kidneys. DOI: 10.52547/ijkd.7472.

本刊更多论文

纳米氧化石墨烯对干细胞治疗的顺铂诱导的急性肾损伤的影响。

引言：石墨烯基纳米材料已显示出一定程度的干细胞保护作用，防止细胞死亡。由于其独特的功能，肾脏会接触到许多有毒物质。另一方面，据报道，干细胞对急性肾损伤（AKI）的治疗作用很小。在此，我们解释了氧化石墨烯（GO）用于提高间充质干细胞（MSC）治疗顺铂诱导的AKI的疗效。30只成年雄性Wistar Albino大鼠分为5组：对照组（未接受任何治疗）、顺铂组（腹膜内接受5 mg/kg顺铂）、假手术组（顺铂注射后第5天腹膜内接受500µL生理盐水）、[顺铂+MSCs]组（顺铂注射液后接受5×106/kg MSCs），和[顺铂+骨髓间充质干细胞+GO]组（在注射顺铂后接受1.5mg/kg的GO+MSCs）。比较不同组的血清肌酐（Cr）和血尿素氮（BUN）水平的生化分析以及肾脏的组织学研究。研究组之间采用单因素方差分析（ANOVA）和Dunnett检验进行比较。结果：GO改善了骨髓间充质干细胞移植对AKI大鼠血清Cr和BUN的影响。与MSCs组相比，它还减少了动物模型中的细胞死亡、透明质铸型和细胞碎片。结论：GO可提高骨髓间充质干细胞移植治疗肾损伤的疗效。DOI:10.52547/ijkd.7472。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian journal of kidney diseases UROLOGY & NEPHROLOGY-

CiteScore

2.50

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The Iranian Journal of Kidney Diseases (IJKD), a peer-reviewed journal in English, is the official publication of the Iranian Society of Nephrology. The aim of the IJKD is the worldwide reflection of the knowledge produced by the scientists and clinicians in nephrology. Published quarterly, the IJKD provides a new platform for advancement of the field. The journal’s objective is to serve as a focal point for debates and exchange of knowledge and experience among researchers in a global context. Original papers, case reports, and invited reviews on all aspects of the kidney diseases, hypertension, dialysis, and transplantation will be covered by the IJKD. Research on the basic science, clinical practice, and socio-economics of renal health are all welcomed by the editors of the journal.