Graphene oxide down-regulates genes of the oxidative phosphorylation complexes in a glioblastoma

IF 2.946 Q3 Biochemistry, Genetics and Molecular Biology

BMC Molecular Biology Pub Date : 2019-01-03 DOI:10.1186/s12867-018-0119-2

Maciej Szmidt, Adrian Stankiewicz, Kaja Urbańska, Sławomir Jaworski, Marta Kutwin, Mateusz Wierzbicki, Marta Grodzik, Beata Burzyńska, Monika Góra, André Chwalibog, Ewa Sawosz

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引用次数: 14

Abstract

Recently different forms of nanographene were proposed as the material with high anticancer potential. However, the mechanism of the suppressive activity of the graphene on cancer development remains unclear. We examined the effect of oxygenated, reduced and pristine graphene on the gene expression in glioblastoma U87 cell line.

Conducting microarrays and RT-qPCR analysis we explored that graphene oxide (rather than reduced graphene oxide and pristine graphene) down-regulates the mRNA expression of mitochondrial oxidative phosphorylation (OXPHOS) nuclear genes of complexes I, III, IV and V. The presented results provide first evidence for the hypothesis that the suppressed growth of GBM can be the consequence of down-regulation of OXPHOS protein expression and decreased ATP level.

We suggest that changes in the expression of OXPHOS genes identified in our study may mediate the anti-proliferative and anti-migratory effects of graphene oxide in glioblastoma cells. However, further investigations with different cell lines, regarding expression, regulation and activity of OXPHOS genes identified in our study is necessary to elucidate the mechanism mediating the anti-proliferative and anti-migratory effects of graphene oxide in glioblastoma cells.

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氧化石墨烯下调胶质母细胞瘤中氧化磷酸化复合物的基因

近年来，不同形式的纳米石墨烯被认为是具有高抗癌潜力的材料。然而，石墨烯抑制癌症发展的机制尚不清楚。我们研究了氧化石墨烯、还原石墨烯和原始石墨烯对胶质母细胞瘤U87细胞系基因表达的影响。通过微阵列和RT-qPCR分析，我们探索了氧化石墨烯(而不是还原的氧化石墨烯和原始的石墨烯)下调复合物I、III、IV和v的线粒体氧化磷酸化(OXPHOS)核基因的mRNA表达。这些结果为GBM生长抑制可能是OXPHOS蛋白表达下调和ATP水平降低的结果的假设提供了第一个证据。我们认为，在我们的研究中发现的OXPHOS基因表达的变化可能介导氧化石墨烯在胶质母细胞瘤细胞中的抗增殖和抗迁移作用。然而，为了阐明氧化石墨烯在胶质母细胞瘤细胞中抗增殖和抗迁移作用的机制，有必要在不同细胞系中进一步研究本研究中发现的OXPHOS基因的表达、调控和活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Molecular Biology 生物-生化与分子生物学

CiteScore

4.80

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： BMC Molecular Biology is an open access journal publishing original peer-reviewed research articles in all aspects of DNA and RNA in a cellular context, encompassing investigations of chromatin, replication, recombination, mutation, repair, transcription, translation and RNA processing and function.