The effect of lunasin from Indonesian soybean extract on histopatologic examination and cox-2 expression in dextran sodium sulfate-induced mice colon.

International journal of physiology, pathophysiology and pharmacology Pub Date : 2018-12-25 eCollection Date: 2018-01-01
Kusmardi Kusmardi, Nessa Nessa, Ari Estuningtyas, Aryo Tedjo
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Abstract

Inflammatory bowel disease (IBD) is a condition describing chronic gastrointestinal inflammation. Chronic inflammation in colon can develop into colon cancer. Lunasin has been known to inhibit inflammatory reactions induced by lipopolysaccharide in vitro. The effect of lunasin to inhibit inflammation in vivo is not widely known. In this study, we analyzed the effect of lunasin from soybean to decrease the risk of inflammation by analyzing histopathologic feature and the expression of COX-2. 30 mice are divided into 6 groups. Normal group was not induced by dextran sodium sulfate (DSS). The other groups were induced by 2% DSS through drinking water for 9 days. After 9 days, negative control group did not receive any treatment. The other groups received treatment given lunasin dose 20 mg/kg body weight (BW) and 40 mg/kg BW, commercial lunasin and positive control given aspirin. Treatment was performed for 5 weeks. Inflammatory colon histopathologic examination and immunohistochemical score of COX-2 proteins were analyzed using statistical tests. Lunasin dose 20 mg/kg BW and 40 mg/kg BW were able to significantly reduce inflammation (P<0.05) performed by histopathologic feature with an average score of 2.52 and 2.16 COX-2 expression decreased significantly (P<0.05) with an average score of 43.674 and 33.349. Lunasin dose 20 mg/kg BW and 40 mg/kg BW were able to inhibit inflammation and decrease the expression of COX-2 in colon induced by DSS.

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印尼大豆提取物lunasin对右旋糖酐硫酸钠诱导的小鼠结肠组织病理学检查和cox-2表达的影响。
炎症性肠病(IBD)是一种描述慢性胃肠道炎症的疾病。结肠慢性炎症可发展为结肠癌。众所周知,Lunasin可以抑制脂多糖在体外诱导的炎症反应。lunasin在体内抑制炎症的作用还不广为人知。在本研究中,我们通过分析组织病理学特征和COX-2的表达,分析了大豆lunasin降低炎症风险的作用。将30只小鼠分为6组。正常组未经右旋糖酐硫酸钠(DSS)诱导。其他组用2%DSS通过饮水诱导9天。9天后,阴性对照组未接受任何治疗。其他组接受lunasin剂量20 mg/kg体重(BW)和40 mg/kg体重的治疗,商业lunasin和阳性对照给予阿司匹林。治疗5周。应用统计学检验分析炎症性结肠组织病理学检查和COX-2蛋白的免疫组织化学评分。Lunasin剂量20mg/kg BW和40mg/kg BW能显著减轻炎症反应(P
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