Evaluation of the protective effects of curcumin-rich turmeric (Curcuma longa) extract against isotretinoin-induced liver damage in rats.

IF 3.2 4区医学 Q1 Pharmacology, Toxicology and Pharmaceutics

Toxicology Mechanisms and Methods Pub Date : 2024-02-01 Epub Date: 2024-01-29 DOI:10.1080/15376516.2023.2260454

Nubar Nuriyeva, Emir Enis Yurdgulu, Abdulmecit Albayrak, Huseyn Aliyev, Kubra Aliyeva, Beyzagul Erkayman, Yasin Bayir

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引用次数: 0

Abstract

Aim: The aim of this study was to evaluate the protective effect of curcumin-rich turmeric (CRT) extract against isotretinoin (ISO)-induced liver damage through routine biochemical parameters and oxidative stress parameters that indicate liver damage.

Material and method: 42 albino Wistar rats of 200 g were randomly grouped as Group I: Healthy control, Group II: Sunflower oil, Group III: Curcumin 200 mg/kg, Group IV: ISO control groups (7.5 mg/kg), Group V: Curcumin 50 mg/kg + ISO 7.5 mg/kg, Group VI: Curcumin 100 mg/kg + ISO 7.5 mg/kg, Group VII: Curcumin 200 mg/kg + ISO 7.5 mg/kg. At the end, after the rats were killed, their blood and liver tissues were collected. ALT and AST levels in serum; superoxide dismutase activity (SOD), GSH, and MDA levels in liver tissue were determined.

Results: Our results showed that ALT, AST, and MDA levels increased, and SOD and GSH levels decreased in the ISO-administered group compared to the healthy control group. CRT 50, 100, and 200 mg/kg groups were compared to ISO group. A dose-dependent increase in protective effect was observed. A decrease in ALT, AST, and MDA levels, and an increase in SOD and GSH levels were determined. A protective effect was found at all doses. The best protective effect was in the CRT 200 mg/kg group.

Conclusion: CRT extract can be considered a candidate herbal medicine for the elimination of liver damage in individuals using ISO. However, further experimental and clinical validation should be studied.

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富含姜黄素的姜黄提取物对异维甲酸诱导的大鼠肝损伤的保护作用评估。

目的：本研究的目的是通过指示肝损伤的常规生化参数和氧化应激参数来评估富含姜黄素的姜黄（CRT）提取物对异维甲酸（ISO）诱导的肝损伤的保护作用。材料和方法：42只白化Wistar大鼠，每只200只 g随机分为第一组：健康对照组，第二组：葵花油组，第三组：姜黄素200 mg/kg，第IV组：ISO对照组（7.5 mg/kg），第V组：姜黄素50 毫克/千克 + ISO 7.5 mg/kg，第VI组：姜黄素100 毫克/千克 + ISO 7.5 mg/kg，第VII组：姜黄素200 毫克/千克 + ISO 7.5 mg/kg。最后，在杀死大鼠后，采集它们的血液和肝组织。血清ALT和AST水平；测定肝组织超氧化物歧化酶（SOD）、谷胱甘肽（GSH）和丙二醛（MDA）水平。结果：与健康对照组相比，ISO给药组的ALT、AST和MDA水平升高，SOD和GSH水平降低。CRT 50、100和200 mg/kg组与ISO组比较。观察到保护作用的剂量依赖性增加。ALT、AST和MDA水平降低，SOD和GSH水平升高。在所有剂量下都发现了保护作用。CRT200的保护效果最好 mg/kg组。结论：CRT提取物可以被认为是一种使用ISO消除个体肝损伤的候选草药。然而，还需要研究进一步的实验和临床验证。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Toxicology Mechanisms and Methods 医学-毒理学

CiteScore

6.60

自引率

3.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Toxicology Mechanisms and Methods is a peer-reviewed journal whose aim is twofold. Firstly, the journal contains original research on subjects dealing with the mechanisms by which foreign chemicals cause toxic tissue injury. Chemical substances of interest include industrial compounds, environmental pollutants, hazardous wastes, drugs, pesticides, and chemical warfare agents. The scope of the journal spans from molecular and cellular mechanisms of action to the consideration of mechanistic evidence in establishing regulatory policy. Secondly, the journal addresses aspects of the development, validation, and application of new and existing laboratory methods, techniques, and equipment. A variety of research methods are discussed, including: In vivo studies with standard and alternative species In vitro studies and alternative methodologies Molecular, biochemical, and cellular techniques Pharmacokinetics and pharmacodynamics Mathematical modeling and computer programs Forensic analyses Risk assessment Data collection and analysis.