Development of an optimized RT-LAMP test for the detection of SARS-CoV-2

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-10-04 DOI:10.1016/j.biologicals.2023.101716

Navid Momenifar, Mohammad Pirouzfar, Zohreh Hashemian, Abdolreza Daneshvar Amoli

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引用次数: 0

Abstract

SARS-COV-2 is the causative agent of an acute respiratory syndrome called Coronavirus disease 2019 (COVID-19) with a varying mortality rate from 2019 to 2022. There are several measures for control and prevention of Covid-19 including using mask, vaccine injections, as well as screening the potential cases. We aimed to design and develop a molecular method (RT-LAMP) for detecting coronavirus in biological samples that is cheaper, faster and easier than conventional molecular methods. In this study, various reaction components were explored to make the optimal combination of an RT-LAMP master mix composition. The results revealed the ability of this RT-LAMP test in specifically identifying 100 copies of mixture of N and E genes in just 30–45 min. This study demonstrated the reliable performance of the RT-LAMP method for the detection of SARS-COV-2 in biological samples. Given the significant advantages of this method compared to the gold standard qRT-PCR, it can be employed as a promising tool for the diagnosis of coronavirus as well as other pathogenic viruses.

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用于检测严重急性呼吸系统综合征冠状病毒2型的优化RT-LAMP测试的开发。

SARS-COV-2是一种被称为2019冠状病毒病（新冠肺炎）的急性呼吸综合征的病原体，2019年至2022年的死亡率各不相同。有几种控制和预防新冠肺炎的措施，包括使用口罩、注射疫苗以及筛查潜在病例。我们旨在设计和开发一种在生物样本中检测冠状病毒的分子方法（RT-LAMP），该方法比传统分子方法更便宜、更快、更容易。在本研究中，对各种反应组分进行了探索，以制备RT-LAMP主混合物组合物的最佳组合。结果揭示了这种RT-LAMP测试在短短30-45分钟内特异性鉴定100个拷贝的N和E基因混合物的能力。本研究证明了RT-LAMP方法检测生物样本中严重急性呼吸系统综合征冠状病毒2型的可靠性能。与金标准qRT-PCR相比，该方法具有显著优势，可作为诊断冠状病毒和其他致病病毒的一种有前途的工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.