Comparison of the effectiveness of various parasitological methods in detecting nematode eggs in different types of soil.

IF 1.3 4区 医学 Q4 ENVIRONMENTAL SCIENCES
Jolanta Małgorzata Zdybel, Jacek Sroka, Jacek Karamon, Ewa Bilska-Zając, Angelina Wójcik-Fatla, Teresa Kłapeć, Piotr Skowron, Grzegorz Siebielec, Tamara Jadczyszyn, Tomasz Cencek
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Abstract

Introduction and objective: Natural fertilizers, sewage sludge, digestates, as well as organic fertilizers produced on their basis, can become a source of parasitological contamination of cultivated land. High concentration of invasive forms of parasites in the soil may pose a threat to human and animal health. Therefore, it is necessary to control the hygienic condition of fertilizers and fertilized soils with particular emphasis on parasites. The aim of the study was to compare the effectiveness of methods commonly used for parasitological examination of soil with own methods which were used to develop the standards.

Material and methods: The study was carried out using samples of sandy soil (SS), horticultural mix soil (HS) and peat-based substrate (PS). Each sample was spiked with 100 dyed Ascaris suum eggs and examined with the use of 6 methods: Vasilkova, Dada, Quinn, and 3 methods according to the Polish Standards (PN-19000, PN- 19005, PN-19006). For each variant, 8 repetitions were made.

Results: The largest number of A. suum eggs were found with PN-19006 (mean number of detected eggs was 21.25, 46.50, 23.00 for HS, SS, PS, respectively. Slightly lower results were obtained using PN-19005 - the mean number eggs was 21.25, 36.00, 16.75, respectively. On the other hand, the mean number of A. suum eggs found with the Dada method was about 2-3 times lower than with the PN-19006 - 15.75, 22.50, 6.50 for HS, SS, PS soil, respectively. Other methods were much less effective.

Conclusions: PN-19006 method turned out to be the most effective in detecting A. suum eggs. This method can be used for parasitological examination of soils and can be the basis for developing a system of methods dedicated to testing different types of soils for the presence of nematode eggs.

不同寄生虫学方法在不同类型土壤中检测线虫卵的有效性比较。
引言和目的:天然肥料、污水污泥、消化物以及在此基础上生产的有机肥料可能成为耕地寄生虫学污染的来源。土壤中高浓度的侵入性寄生虫可能对人类和动物健康构成威胁。因此,有必要控制肥料和施肥土壤的卫生条件,特别是寄生虫。本研究的目的是比较土壤寄生虫学检查常用方法与制定标准所用方法的有效性。材料和方法:采用沙质土壤(SS)、园艺混合土(HS)和泥炭基质(PS)样品进行研究。每个样品掺入100个染色的蛔虫卵,并根据波兰标准(PN-19000、PN-19005、PN-19006)使用6种方法进行检查:Vasilkova、Dada、Quinn和3种方法。对于每个变体,进行8次重复。结果:用PN-19006发现的A.suum卵数量最多(HS、SS和PS的平均检测卵数分别为21.25、46.50和23.00。使用PN-19005获得的结果略低,平均卵数分别是21.25、36.00和16.75。另一方面,使用Dada方法发现的A.suum卵数比PN-19006低约2-3倍,HS、SS、PS土壤分别为15.75、22.50和6.50效果要差得多。结论:PN-19006法是最有效的毒鸡蛋检测方法。该方法可用于土壤的寄生虫学检查,并可作为开发一套专门测试不同类型土壤是否存在线虫卵的方法系统的基础。
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来源期刊
Annals of Agricultural and Environmental Medicine
Annals of Agricultural and Environmental Medicine 医学-公共卫生、环境卫生与职业卫生
CiteScore
3.00
自引率
5.90%
发文量
58
审稿时长
4-8 weeks
期刊介绍: All papers within the scope indicated by the following sections of the journal may be submitted: Biological agents posing occupational risk in agriculture, forestry, food industry and wood industry and diseases caused by these agents (zoonoses, allergic and immunotoxic diseases). Health effects of chemical pollutants in agricultural areas , including occupational and non-occupational effects of agricultural chemicals (pesticides, fertilizers) and effects of industrial disposal (heavy metals, sulphur, etc.) contaminating the atmosphere, soil and water. Exposure to physical hazards associated with the use of machinery in agriculture and forestry: noise, vibration, dust. Prevention of occupational diseases in agriculture, forestry, food industry and wood industry. Work-related accidents and injuries in agriculture, forestry, food industry and wood industry: incidence, causes, social aspects and prevention. State of the health of rural communities depending on various factors: social factors, accessibility of medical care, etc.
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