The Antibacterial and Antifungal Activity of Chlorhexidine Diacetate Incorporated into Acrylic Resins Used in Provisional Restorations.

IF 1.4 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Acta Stomatologica Croatica Pub Date : 2023-09-01 DOI:10.15644/asc57/3/4

Secil Ozkan Ata, Canan Akay, Emre Mumcu, Demet Erdonmez

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引用次数: 0

Abstract

Objective: The surface of provisional restorations applied before conventional or implant- supported fixed restorations may cause bacterial or fungal biofilm formation. The aim of this study was to evaluate the antimicrobial activity of acrylic resins used in provisional restorations modified with chlorhexidine diacetate.

Methods: 120 cylindrical, auto-polymerized resin samples modified with chlorhexidine diacetate were prepared at concentrations of 0 (control), 1, 3, 5 wt %. The antimicrobial activity was examined against Streptococcus mutans, Enterococcus faecalis, and Candida albicans using Crystal Violet quantification, MTT assay, and Scanning Electron Microscopy. Data were analyzed by ANOVA and paired sample t-tests (α=0.05).

Results: The addition of chlorhexidine diacetate influenced the growth rate and metabolic activity of microorganisms. The antimicrobial effect against C. albicans and S. mutans statistically increased with the percentage of chlorhexidine diacetate. E. faecalis bacteria were less affected by chlorhexidine diacetate compared to other pathogens.

Conclusion: It has been shown that the effectiveness of CHDA in inhibiting the proliferation of microorganisms correlated positively with increasing concentration levels. More research is needed to confirm the impact of different chlorhexidine concentrations on the mechanical properties, clinical efficacy, and antimicrobial properties of CDHA.

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二乙酸氯己定掺入丙烯酸树脂用于临时修复的抗菌和抗真菌活性。

目的：在常规或种植体支持的固定修复体之前，临时修复体的表面可能会导致细菌或真菌生物膜的形成。本研究的目的是评估用二乙酸氯己定修饰的临时修复体中使用的丙烯酸树脂的抗菌活性。方法：制备120个用二乙酸氯己定改性的圆柱形自聚合树脂样品，浓度分别为0（对照）、1、3、5wt%。采用结晶紫定量法、MTT法和扫描电子显微镜检测了对变形链球菌、粪肠球菌和白色念珠菌的抗菌活性。通过方差分析和配对样本t检验（α=0.05）对数据进行分析。结果：双乙酸氯己定的添加影响了微生物的生长速率和代谢活性。对白色念珠菌和变形链球菌的抗菌效果随着二乙酸氯己定的百分比而增加，具有统计学意义。与其他病原体相比，粪便大肠杆菌受二乙酸氯己定的影响较小。结论：CHDA抑制微生物增殖的效果与浓度的增加呈正相关。需要更多的研究来证实不同浓度的氯己定对CDHA的机械性能、临床疗效和抗菌性能的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Stomatologica Croatica DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

2.40

自引率

28.60%

发文量

审稿时长

12 weeks

期刊介绍： The Acta Stomatologica Croatica (ASCRO) is a leading scientific non-profit journal in the field of dental, oral and cranio-facial sciences during the past 44 years in Croatia. ASCRO publishes original scientific and clinical papers, preliminary communications, case reports, book reviews, letters to the editor and news. Review articles are published by invitation from the Editor-in-Chief by acclaimed professionals in distinct fields of dental medicine. All manuscripts are subjected to peer review process.

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