TRPM8 as a Potential Biomarker and Therapeutic Target for Gastric Cancer Identified by a Combination of Text Mining and RNA Sequencing.

IF 3.8 4区医学 Q2 GENETICS & HEREDITY

Current gene therapy Pub Date : 2023-01-01 DOI:10.2174/1566523223666230529142423

Na Kong, Wendong Li, Jun Zhang, Xin Wang, Lin Hu, Qiqi Xu

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引用次数: 0

Abstract

Introduction: Gastric cancer is a well-known malignant tumor that causes millions of deaths worldwide every year. Due to the lack of a specific biomarker for gastric cancer, most patients are diagnosed at an advanced stage of the disease which results in a poor prognosis and a higher death rate. Therefore, novel biomarkers are urgently needed for early diagnosis and to improve the survival rate.

Methods: In this study, we conducted RNA sequencing of tumor samples from 21 patients with gastric cancer. A total of 3192 differentially expressed genes (1589 up-regulated and 1603 down-regulated) were identified. Subsequently, we applied a text-mining algorithm for further analysis of these data and selected 30 representative genes to investigate as candidates for novel biomarkers in gastric cancer.

Results: Among these genes, we confirmed transient receptor potential melastatin 8 channels (TRPM8) as a novel biomarker based on Western blot and immunochemistry validation performed on 134 samples. Compared to normal gastric tissue, the tumor tissues exhibited a significantly higher expression level of TRPM8.

Conclusion: This study provides insights into the underlying role of TRPM8 in cell proliferation. In addition, TRPM8 may be used as a potential therapeutic target for patients with gastric cancer.

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通过文本挖掘和RNA测序相结合鉴定TRPM8作为癌症的潜在生物标志物和治疗靶点。

简介：癌症是一种众所周知的恶性肿瘤，每年导致全球数百万人死亡。由于缺乏癌症的特异性生物标志物，大多数患者被诊断为疾病晚期，这导致预后不良和死亡率较高。因此，迫切需要新的生物标志物来进行早期诊断和提高生存率。方法：对21例癌症患者肿瘤标本进行RNA测序。共鉴定出3192个差异表达基因（1589个上调，1603个下调）。随后，我们应用文本细化算法对这些数据进行进一步分析，并选择30个具有代表性的基因作为癌症新生物标志物的候选进行研究。结果：在这些基因中，基于对134个样本进行的Western印迹和免疫化学验证，我们确认了瞬时受体电位美他丁8通道（TRPM8）是一种新的生物标志物。与正常胃组织相比，肿瘤组织表现出显著更高的TRPM8表达水平。结论：本研究为TRPM8在细胞增殖中的潜在作用提供了见解。此外，TRPM8可作为癌症患者的潜在治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current gene therapy 医学-遗传学

CiteScore

6.70

自引率

2.80%

发文量

期刊介绍： Current Gene Therapy is a bi-monthly peer-reviewed journal aimed at academic and industrial scientists with an interest in major topics concerning basic research and clinical applications of gene and cell therapy of diseases. Cell therapy manuscripts can also include application in diseases when cells have been genetically modified. Current Gene Therapy publishes full-length/mini reviews and original research on the latest developments in gene transfer and gene expression analysis, vector development, cellular genetic engineering, animal models and human clinical applications of gene and cell therapy for the treatment of diseases. Current Gene Therapy publishes reviews and original research containing experimental data on gene and cell therapy. The journal also includes manuscripts on technological advances, ethical and regulatory considerations of gene and cell therapy. Reviews should provide the reader with a comprehensive assessment of any area of experimental biology applied to molecular medicine that is not only of significance within a particular field of gene therapy and cell therapy but also of interest to investigators in other fields. Authors are encouraged to provide their own assessment and vision for future advances. Reviews are also welcome on late breaking discoveries on which substantial literature has not yet been amassed. Such reviews provide a forum for sharply focused topics of recent experimental investigations in gene therapy primarily to make these results accessible to both clinical and basic researchers. Manuscripts containing experimental data should be original data, not previously published.